Gene Expression Data
Assay Details
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Assay
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Reference:
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J:180844
Strauss TJ, et al., GATA-like protein-1 (GLP-1) is required for normal germ cell development during embryonic oogenesis. Reproduction. 2011 Feb;141(2):173-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5906821
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Gene symbol:
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Ddx4
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Gene name:
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DEAD box helicase 4
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Antibody:
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Anti-DDX4/MVH (ab13840)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3B E14.5 WT:
embryonic day 14.5
(more  )
Specimen
3B E14.5 WT:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B E14.5 GLP-1 null:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Zglp1tm1Eem/Zglp1tm1Eem
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Age:
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embryonic day 14.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of ovary |
Present |
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3B MVH E14.5 GLP-1 null
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Expression similar between wild-type and GLP-1 null mice at E14.5.
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Specimen
3B E16.5 WT:
embryonic day 16.5
(more )
Specimen
3B E16.5 WT:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B E16.5 GLP-1 null:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Zglp1tm1Eem/Zglp1tm1Eem
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: germ cell of ovary |
Weak |
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3B MVH E16.5 GLP-1 null
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Expression decreases in mutant embryos concomittant with germ cells.
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Specimen
3B E18.5 WT:
embryonic day 18.5
(more )
Specimen
3B E18.5 WT:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B E18.5 GLP-1 null:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Zglp1tm1Eem/Zglp1tm1Eem
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Age:
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embryonic day 18.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: germ cell of ovary |
Weak |
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3B MVH E18.5 GLP-1 null
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Expression decreases in mutant embryos concomittant with germ cells.
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Specimen
3B PD1 WT:
postnatal day 1
(more )
Specimen
3B PD1 WT:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 1
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B PD1 GLP-1 null:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Zglp1tm1Eem/Zglp1tm1Eem
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Age:
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postnatal day 1
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Absent |
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3B MVH PD1 GLP-1 null
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Germ cell loss is completed by birth.
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Analysis Tools