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Gene Expression Data
Assay Details
Assay
Reference: J:240500 Lopez-Domenech G, et al., Loss of Dendritic Complexity Precedes Neurodegeneration in a Mouse Model with Disrupted Mitochondrial Distribution in Mature Dendrites. Cell Rep. 2016 Oct 04;17(2):317-327
Assay type: Western blot
MGI Accession ID: MGI:5906569
Gene symbol: Rhot1
Gene name: ras homolog family member T1
Antibody: anti-Miro1
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Antibody recognizes both Miro1 (Rhot1; duplex at 71-73 kDa) and Miro2 (Rhot2; 74 kDa). Both genes have been annotated.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 71 kDa (a) Genetic BackgroundMutant Allele(s)Sex Note
WT 1 month P m 1 TS28: hippocampus Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT 4 months P m 4 TS28: hippocampus Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT 8 months P m 8 TS28: hippocampus Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT 12 months P m 12 TS28: hippocampus Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
Miro1cko 1 month P m 1 TS28: hippocampus Weak (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko 4 months P m 4 TS28: hippocampus Weak (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko 8 months P m 8 TS28: hippocampus Weak (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko 12 months P m 12 TS28: hippocampus Weak (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Notes:
(a) Duplex at 71-73 kDa.
(b) At one month of age, Miro1 deletion is already significant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory