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Gene Expression Data
Assay Details
Assay
Reference: J:240500 Lopez-Domenech G, et al., Loss of Dendritic Complexity Precedes Neurodegeneration in a Mouse Model with Disrupted Mitochondrial Distribution in Mature Dendrites. Cell Rep. 2016 Oct 04;17(2):317-327
Assay type: Western blot
MGI Accession ID: MGI:5906566
Gene symbol: Rhot1
Gene name: ras homolog family member T1
Antibody: anti-Miro1
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Antibody recognizes both Miro1 (Rhot1; duplex at 71-73 kDa) and Miro2 (Rhot2; 74 kDa). Both genes have been annotated.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 71 kDa (a) Genetic BackgroundMutant Allele(s)Sex Note
WT (-Cre) Ctx P adult TS28: cerebral cortex Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT (-Cre) Hipp P adult TS28: hippocampus Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
WT (-Cre) Cereb P adult TS28: cerebellum Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)WtsiNot Specified
Miro1cko Ctx P adult TS28: cerebral cortex Weak (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko Hipp P adult TS28: hippocampus Weak (b) involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Miro1cko Cereb P adult TS28: cerebellum Present involves: C57BL/6Dnk * C57BL/6N * FVB/N Rhot1tm1c(EUCOMM)Wtsi/Rhot1tm1c(EUCOMM)Wtsi, Tg(Camk2a-cre)1Gsc/0Not Specified Conditional mutant.
Notes:
(a) Duplex at 71-73 kDa.
(b) Specific deletion occurred primarily in adult principal neurons of the cortex and hippocampus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory