Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:18836
Hankin MH, et al., Cell adhesion molecules in the early developing mouse retina: retinal neurons show preferential outgrowth in vitro on L1 but not N-CAM. J Neurobiol. 1994 May;25(5):472-87
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5906246
|
|
Gene symbol:
|
L1cam
|
|
Gene name:
|
L1 cell adhesion molecule
|
|
Antibody:
|
anti-L1
|
|
Detection system:
|
horseradish peroxidase
|
|
|
Results
|
Specimen
2A:
embryonic day 13.5
(more  )
Specimen
2A:
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neural retina |
Present |
Regionally restricted |
2A
|
Most intense expression along inner margin, corresponding to developing optic fiber layer, and optic disc, continuous with optic nerve. Very faint expression in neuroblastic cell layer immedicately adjacent to optic fiber layer.
|
Specimen
3:
embryonic day 15.5
(more )
Specimen
3:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina nerve fiber layer |
Very strong |
Regionally restricted |
3
|
Expression significantly more intense, extending from central to peripheral retina. Expression in optic fibre layer and optic nerve than at E13
|
| TS23: retina nuclear layer |
Strong |
Regionally restricted |
3
|
Stronger expression in neuroblast layer, extending farther toward the outer retina.
|
Specimen
4A:
embryonic day 13.5
(more )
Note: Intraorbital portion of optic nerve.
Specimen
4A:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Intraorbital portion of optic nerve.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: optic nerve |
Present |
Regionally restricted |
4A
|
Strong expression over entire course of optic nerve. Expression in intraorbital portion consolidated almost exclusively in ventral zone. Expression in axon bundles located primarily in ventral part of optic nerve.
|
Specimen
4B:
embryonic day 13.5
(more )
Note: Mid-nerve region of optic nerve.
Specimen
4B:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Mid-nerve region of optic nerve.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: optic nerve |
Present |
Regionally restricted |
4B
|
Expression in axon bundles located primarily in ventral part of optic nerve. In mid-nerve region, a few fascicles have moved toward the dorsal region.
|
Specimen
4C:
embryonic day 13.5
(more )
Note: Intracranial portion of optic nerve.
Specimen
4C:
(close )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Intracranial portion of optic nerve.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: optic nerve |
Present |
Regionally restricted |
4C
|
Expression in axon bundles located primarily in ventral part of optic nerve. Some fascles reach most dorsal aspect in intracranial region.
|
|
Analysis Tools