Probe:	IMAGE clone 3497841
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: telencephalon mantle layer	Present		1A
TS22: amygdala	Present		1A
TS22: basal forebrain	Present		1A
TS22: corpus striatum	Present		1A
TS22: hippocampus	Present		1A
TS22: neocortex	Present		1A
TS22: hindbrain mantle layer	Present		1A
TS22: midbrain mantle layer	Present		1A
TS22: glossopharyngeal ganglion	Strong		1A	Strong expression in cranial nerve ganglia.
TS22: trigeminal ganglion	Strong		1A	Strong expression in cranial nerve ganglia.
TS22: vestibulo-cochlear ganglion	Strong		1A	Strong expression in cranial nerve ganglia.
TS22: ventricular layer	Absent		1A	No expression in ventricular zone (neuroepithelium) of central nervous system.
TS22: lung	Present		1A
TS22: limb cartilage condensation	Present		1A
TS22: sternum	Present		1A	Expression in cartilage condensations of sternum.
TS22: alimentary system	Present		1A

Structure	Level	Pattern	Image	Note
TS22: telencephalon mantle layer	Present		1B
TS22: cerebral cortex	Present	Regionally restricted	1B	Specific expression throughout the preplate, particularly at inner and outer edges.
TS22: hippocampus	Present		1B
TS22: diencephalon mantle layer	Present		1B
TS22: prethalamus	Strong		1B	High levels of expression in ventral thalamus.
TS22: hypothalamus	Strong	Regionally restricted	1B	Expression in caudal portions of hypothalamus.
TS22: dorsal thalamus	Ambiguous		1B	Authors report dorsal thalamus almost completely devoid of expression.
TS22: midbrain mantle layer	Present		1B	Expression in large areas of midbrain mantle.
TS22: inferior colliculus	Present		1B	Expression in tectum in layers.
TS22: hindbrain mantle layer	Present		1B
TS22: cerebellum	Strong	Regionally restricted	1B
TS22: pons	Strong	Regionally restricted	1B
TS22: hindbrain	Present	Regionally restricted	1B	Expression around 4th ventricle (not ventricular zone).
TS22: brainstem	Present	Widespread	1B
TS22: spinal cord	Present	Widespread	1B
TS22: ventricular layer	Absent		1B	No expression in ventricular zone (neuroepithelium) of central nervous system
TS22: dorsal root ganglion	Strong		1B
TS22: limb cartilage condensation	Present		1B
TS22: sternum	Present		1B
TS22: lung	Present		1B
TS22: alimentary system	Present		1B	Expression in digestive tract.

Structure	Level	Pattern	Image	Note
TS22: telencephalon mantle layer	Present		1C
TS22: telencephalon septum	Present		1C
TS22: cerebral cortex	Present	Regionally restricted	1C	Specific expression throughout the preplate, particularly at inner and outer edges.
TS22: main olfactory bulb	Strong	Regionally restricted	1C	Intense expression through the entire differentiating mantle.
TS22: diencephalon mantle layer	Present		1C
TS22: prethalamus	Strong		1C	High levels of expression in ventral thalamus.
TS22: hypothalamus	Strong	Regionally restricted	1C	High levels of expression in caudal portions of hypothalamus.
TS22: dorsal thalamus	Ambiguous	Regionally restricted	1C
TS22: midbrain mantle layer	Present		1C
TS22: superior colliculus	Present		1C	Expression in tectum in layers.
TS22: midbrain tegmentum	Present		1C
TS22: hindbrain mantle layer	Present		1C
TS22: brainstem	Present	Widespread	1C
TS22: spinal cord	Present	Widespread	1C
TS22: ventricular layer	Absent		1C	No expression in ventricular zone (neuroepithelium) of central nervous system
TS22: dorsal root ganglion	Strong		1C
TS22: nasal epithelium	Present		1C
TS22: gut	Present		1C

Structure	Level	Pattern	Image	Note
TS22: telencephalon mantle layer	Present		1D
TS22: telencephalon septum	Present		1D
TS22: brainstem	Present	Widespread	1D
TS22: spinal cord	Present	Widespread	1D
TS22: vertebral cartilage condensation	Present		1D
TS22: esophagus	Present		1D	Expression in digestive tract, including esophagus

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	1E	Specific expression throughout the preplate, particularly at inner and outer edges.
TS22: diencephalon mantle layer	Present		1E
TS22: hindbrain mantle layer	Present		1E
TS22: midbrain mantle layer	Present		1E
TS22: epithalamus	Present		1E
TS22: hypothalamus	Present	Regionally restricted	1E	Expression in anterior hypothalamic area.
TS22: cerebellum	Present		1E
TS22: mammillary area	Present		1E
TS22: tectum	Present		1E

Structure	Level	Pattern	Image	Note
TS22: cochlear ganglion	Present		1F	In developing cochlear system, expression confined to eighth cranial nerve ganglion.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		1G

Structure	Level	Pattern	Image	Note
TS22: lung	Strong	Regionally restricted	1H	Intense expression around some of larger bronchi

Structure	Level	Pattern	Image	Note
TS22: alimentary system	Present	Regionally restricted	1I	Expression in wall of digestive tract.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present		2J
TS22: hypothalamus	Present	Regionally restricted	2J	Expression in anterior hypothalamic area
TS22: mammillary area	Present		2J
TS22: preoptic area	Present		2J

Structure	Level	Pattern	Image	Note
TS22: tectum	Strong	Regionally restricted	2K
TS22: midbrain ventricular layer	Absent		2K	In the midbrain, no expression in neuroepithelium (ventricular zone).

Structure	Level	Pattern	Image	Note
TS22: cerebellum mantle layer	Present		2L
TS22: cerebellum	Present	Regionally restricted	2L	Expression in deep cortical nuclei.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Scattered		Expression in abundant, scattered cells in brainstem and spinal cord.
TS22: spinal cord	Present	Scattered		Expression in abundant, scattered cells in brainstem and spinal cord.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong		3A
TS28: cortical layer V	Strong		3A
TS28: cortical layer II	Weak		3A
TS28: cortical layer III	Weak		3A
TS28: piriform cortex	Strong		3A
TS28: basal ganglia	Present	Scattered	3A
TS28: caudate-putamen	Present	Scattered	3A
TS28: globus pallidus	Present	Scattered	3A
TS28: basal forebrain	Moderate	Widespread	3A
TS28: anterior dorsal thalamic nucleus	Present		3A	Regional differential expresion in hippocampal and thalamic subregions.
TS28: medial dorsal thalamic nucleus	Present		3A	Regional differential expresion in hippocampal and thalamic subregions.
TS28: nucleus reuniens	Present		3A	Regional differential expresion in hippocampal and thalamic subregions.
TS28: reticular thalamic nucleus	Present		3A	Regional differential expresion in hippocampal and thalamic subregions.
TS28: hippocampus CA1	Present		3A
TS28: hippocampus CA3	Strong		3A	Expression most intense in CA3.
TS28: hippocampus	Present	Scattered	3A	Expression in scattered hilar cells.
TS28: hippocampus stratum oriens	Present		3A
TS28: dentate gyrus	Absent		3A

Structure	Level	Pattern	Image	Note
TS28: lateral habenular nucleus	Strong		3B
TS28: medial habenular nucleus	Present		3B
TS28: paraventricular thalamic nucleus	Present		3B	Medium intensity expression in periventricular thalamic nucleus.