Gene Expression Data
Assay Details
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Assay
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Reference:
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J:137794
Trevant B, et al., Expression of secreted frizzled related protein 1, a Wnt antagonist, in brain, kidney, and skeleton is dispensable for normal embryonic development. J Cell Physiol. 2008 Oct;217(1):113-26
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5905420
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Gene symbol:
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Sfrp1
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Gene name:
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secreted frizzled-related protein 1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative 2% paraformaldehyde with 0.2% glutaraldehyde.
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Results
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Specimen
2 E11.5:
(close  )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain |
Present |
Regionally restricted |
2A E11.5
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Expression is in the frontal brain.
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| TS19: ear |
Present |
Regionally restricted |
2A E11.5
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Expression is in focal regions.
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| TS19: eye |
Present |
Regionally restricted |
2A E11.5
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Expression is in focal regions.
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| TS19: somite |
Present |
Regionally restricted |
2A E11.5
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Expression is in the caudal somites.
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| TS19: body cavity or lining |
Present |
Regionally restricted |
2A E11.5
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Expression is in the lower abdominal cavity.
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| TS19: future spinal cord |
Weak |
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2A E11.5
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Specimen
2 E13.5:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brain |
Present |
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2A E13.5
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| TS21: eye |
Present |
|
2A E13.5
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| TS21: somite |
Present |
Regionally restricted |
2A E13.5
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Expression is in the caudal somites.
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| TS21: body cavity or lining |
Present |
Regionally restricted |
2A E13.5
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Expression is in the abdominal cavity.
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| TS21: bone |
Present |
Regionally restricted |
2A E13.5
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Expression is in the epiphysis of the long bones.
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Specimen
2 E15.5 left:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
2 E15.5 middle:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
2 E15.5 right:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3Aa:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: limb |
Strong |
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3Aa
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Specimen
3Ab:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: femur |
Strong |
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3Ab
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Specimen
3Ac:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
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|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: rib |
Strong |
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3Ac
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Expression is strongest at the growth plates.
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| TS25: vertebra |
Strong |
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3Ac
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Specimen
3Ad:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
|
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
|
Fixation:
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Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: rib |
Strong |
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3Ad
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Expression is strongest at the growth plates.
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| TS25: vertebra |
Strong |
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3Ad
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Specimen
3Ae:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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Specimen
3Af:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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Specimen
3Ag:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
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Age:
|
postnatal newborn
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: chondrocranium |
Weak |
Regionally restricted |
3Ah
,
3Ag
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Expression is near the suture lines.
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Specimen
3Ai:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: nasal bone |
Strong |
|
3Ai
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| TS27: eye |
Present |
Regionally restricted |
3Ai
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Expression is in the outer eye.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
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Age:
|
postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cartilage |
Present |
Regionally restricted |
3B right panel
,
3B
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Expression is in regions near the articular surface and near tendon insertion.
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Specimen
4a:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Strong |
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4a
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Specimen
4b:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4d:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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Specimen
4e:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
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Age:
|
postnatal day 3
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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Specimen
4g:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
|
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Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal day 3
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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Specimen
4h:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal day 3
|
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Sex:
|
Not Specified
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Type:
|
section
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|
Fixation:
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Other - See Notes
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Embedding:
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Cryosection
|
Specimen
5A:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
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Specimen
5B:
(close )
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Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal week 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
7A bottom left:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: rib |
Very strong |
Regionally restricted |
7A beta-Gal bottom left
|
Expression is in areas undergoing endochondrial ossification.
|
Specimen
7A bottom right:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: rib |
Very strong |
Regionally restricted |
7A beta-Gal bottom right
|
Expression is in areas of cortical bone formation.
|
Specimen
7B:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: rib |
Very strong |
|
7B beta-Gal
,
7C top left
|
Expression is in the areas of bone formation.
|
| TS27: vertebra |
Very strong |
|
7B beta-Gal
,
7C top left
|
Expression is in the areas of bone formation.
|
| TS27: chondrocranium |
Very strong |
Regionally restricted |
7B beta-Gal
,
7C bottom left
|
Expression is in the intramembraneous craniofacial bone in the areas of bone formation.
|
| TS27: mandible |
Very strong |
Regionally restricted |
7B beta-Gal
,
7C bottom left
|
Expression is in the intramembraneous craniofacial bone in the areas of bone formation.
|
| TS27: maxilla |
Very strong |
Regionally restricted |
7B beta-Gal
,
7C bottom left
|
Expression is in the intramembraneous craniofacial bone in the areas of bone formation.
|
Specimen
8A:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8B:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
10B:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: femur |
Present |
Regionally restricted |
10Bb
,
10Ba
,
10Bc
,
10Bd
|
Expression is in osteoclasts.
|
Specimen
not shown E14.5:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
not shown newborn:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
not shown P w 1:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal week 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
not shown P m 2:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal month 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
Specimen
not shown P m 3:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sfrp1tm1Bsk/Sfrp1tm1Bsk
|
|
Age:
|
postnatal month 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
|
Analysis Tools
)