Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Esr2tm1.1(cre)Hze
Name:	estrogen receptor 2 (beta); targeted mutation 1.1, Hongkui Zeng
MGI ID:	MGI:5904044
Synonyms:	Esr2-IRES2-Cre-D
Gene:	Esr2  Location: Chr12:76167193-76224033 bp, - strand  Genetic Position: Chr12, 33.52 cM, cytoband D1-D3
Alliance:	Esr2tm1.1(cre)Hze page

Germline Transmission:	Earliest citation of germline transmission: J:146821
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Esr2tm1.1(cre)Hze expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Esr2 (MGI:109392)   Mutation details: The targeting vector contained, from 5' to 3', a partial Esr2 sequence spanning exon 10 (including the endogenous stop codon), an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::FRT5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). The resulting Esr2-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice. Cre recombinase expression/activity largely recapitulates the endogenous Esr2 gene expression in the brain - observed mainly in medial amygdalar nucleus, bed nuclei of the stria terminalis, paraventricular hypothalamic nucleus, cortical layer 6 and scattered neurons elsewhere in the brain. (J:146821)

Activity:	Tissue activity of this recombinase allele
Driver:	Esr2 (mouse) Summary of all recombinase alleles driven by Esr2. Alliance mouse Esr2 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Esr2 Mutation:	32 strains or lines available

Original:	J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06;
All:	7 reference(s)