Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Zap70tm3.1Weis
Name:	zeta-chain (TCR) associated protein kinase; targeted mutation 3.1, Arthur Weiss
MGI ID:	MGI:5903620
Synonyms:	Zap70 W131A
Gene:	Zap70  Location: Chr1:36800879-36821899 bp, + strand  Genetic Position: Chr1, 15.41 cM, cytoband A4-C1
Alliance:	Zap70tm3.1Weis page

Germline Transmission:	Earliest citation of germline transmission: J:241231
Parent Cell Line:	PRX-B6T (ES Cell)
Strain of Origin:	C57BL/6J

Allele Type:    Targeted (Hypomorph) Mutation:    Nucleotide substitutions   Mutation details: A W131A (TGG->GCG, tryptophan to alanine) mutation was introduced into exon 2 and a neomycin cassette (flanked by loxP sites and inserted in reverse transcriptional orientation) was placed in intron 3. Cre-mediated recombination removed the floxed neo cassette. The mutation, located in interdomain A reduces protein expression by 50% in thymocytes and peripheral T cells. (J:241231)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Zap70 Mutation:	55 strains or lines available

Original:	J:241231 Hsu LY, et al., Destabilizing the autoinhibitory conformation of Zap70 induces up-regulation of inhibitory receptors and T cell unresponsiveness. J Exp Med. 2017 Mar 06;214(3):833-849
All:	2 reference(s)