Antibody:	270 antibody
Detection system:	Secondary antibody
Assay notes:	Did authors did not specify which antibody was used, the 859 antibody (MGI:5902851) may have been used as well.

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present		2a_E10.5 Eye , 2a_E10.5 Eye (mag)	Expression is at the outer peripheral area of the inner part of the optic cup.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	2a_E10.5 Brain , 2a_E10.5 Brain (mag)	Expression is at the external limiting membrane in glial cells.

Structure	Level	Pattern	Image	Note
TS19: neural retina	Strong	Regionally restricted	2a_E11.5 Eye , 2a_E11.5 Eye (mag)	Expression is in the area of the photoreceptors facing the future pigment epithelium.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2a_E11.5 Brain , 2a_E11.5 Brain (mag)	Expression is in the anterior ventral area.
TS19: skeleton	Present	Regionally restricted	2a_E11.5 Brain , 2a_E11.5 Brain (mag)	Expression is in tissue that will develop into cartilage at the base of the skull.

Structure	Level	Pattern	Image	Note
TS19: peripheral nerve trunk	Present		2a_E11.5 Peripheral Nerve Trunk

Structure	Level	Pattern	Image	Note
TS20: neural retina	Strong	Regionally restricted	2a_E12.5 Eye , 2a_E12.5 Eye (mag)	Expression is in the precursors of the photoreceptors.
TS20: optic nerve	Weak	Regionally restricted	2a_E12.5 Eye , 2a_E12.5 Eye (mag)	Expression is in fibers at the inner side of the optic nerve.
TS20: lens vesicle	Present	Regionally restricted	2a_E12.5 Eye (mag) , 2a_E12.5 Eye	Expression is in cells at the periphery of the lens and in the lens fibers.
TS20: cornea	Present	Single cells	2a_E12.5 Eye (mag) , 2a_E12.5 Eye

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2a_E12.5 Brain , 2a_E12.5 Brain (mag)	Expression is in the medial part.
TS20: brain	Present	Regionally restricted	2a_E12.5 Brain , 2a_E12.5 Brain (mag)	Expression is in both hemispheres in fiber-like structures.
TS20: cortical plate	Absent		2a_E12.5 Brain (mag) , 2a_E12.5 Brain

Structure	Level	Pattern	Image	Note
TS20: peripheral nerve trunk	Present		2a_E12.5 Peripheral Nerve Trunk

Structure	Level	Pattern	Image	Note
TS21: choroidal vasculature	Present		2a_E13.5 Eye , 2a_E13.5 Eye (mag)
TS21: lens fiber	Present		2a_E13.5 Eye , 2a_E13.5 Eye (mag)
TS21: eye mesenchyme	Present	Regionally restricted	2a_E13.5 Eye , 2a_E13.5 Eye (mag)	Expression is in the connective tissue.
TS21: cornea	Present	Single cells	2a_E13.5 Eye (mag) , 2a_E13.5 Eye

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	2a_E13.5 Brain (mag) , 2a_E13.5 Brain	Expression is in fibers under the cortical plate and is absent in the cortical neurons.

Structure	Level	Pattern	Image	Note
TS21: peripheral nerve trunk	Present		2a_E13.5 Peripheral Nerve Trunk

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Present		2a_E14.5 Eye , 2a_E14.5 Eye (mag)
TS22: vitreous humour	Present		2a_E14.5 Eye , 2a_E14.5 Eye (mag)
TS22: cornea	Weak	Regionally restricted	2a_E14.5 Eye , 2a_E14.5 Eye (mag)	Expression is in most of the cornea.
TS22: eye	Present	Regionally restricted	2a_E14.5 Eye (mag) , 2a_E14.5 Eye	Expression is in the sclera.
TS22: retina	Absent		2a_E14.5 Eye , 2a_E14.5 Eye (mag)

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	2a_E14.5 Brain (mag) , 2a_E14.5 Brain	There is dispersed expression in glial cells throughout the brain especially in the midbrain and pons areas.
TS22: midbrain	Present	Regionally restricted	2a_E14.5 Brain (mag) , 2a_E14.5 Brain	There is dispersed expression in glial cells throughout the brain especially in the midbrain and pons areas.
TS22: pons	Present	Regionally restricted	2a_E14.5 Brain (mag) , 2a_E14.5 Brain	There is dispersed expression in glial cells throughout the brain especially in the midbrain and pons areas.

Structure	Level	Pattern	Image	Note
TS22: peripheral nerve trunk	Present		2a_E14.5 Peripheral Nerve Trunk

Structure	Level	Pattern	Image	Note
TS23: choroidal vasculature	Present		2a_E15.5 Eye (mag) , 2a_E15.5 Eye
TS23: lens anterior epithelium	Absent		2a_E15.5 Eye (mag) , 2a_E15.5 Eye
TS23: retina	Absent		2a_E15.5 Eye (mag) , 2a_E15.5 Eye

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	2a_E15.5 Brain , 2a_E15.5 Brain (mag)	Expression is in nerve fibers.
TS23: cerebral cortex	Weak		2a_E15.5 Brain , 2a_E15.5 Brain (mag)

Structure	Level	Pattern	Image	Note
TS23: peripheral nerve trunk	Present		2a_E15.5 Peripheral Nerve Trunk

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Present		2a_E16.5 Eye (mag) , 2a_E16.5 Eye
TS24: optic choroid	Present		2a_E16.5 Eye (mag) , 2a_E16.5 Eye
TS24: retina layer	Present	Regionally restricted	2a_E16.5 Eye (mag) , 2a_E16.5 Eye	Expression is in the cell bodies and fibers of the ganglion cell layer and is absent in the photoreceptors and bipolar layer.
TS24: pigmented retinal epithelium	Absent		2a_E16.5 Eye (mag) , 2a_E16.5 Eye

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted	2a_E16.5 Brain (mag) , 2a_E16.5 Brain	Expression is throughout the brain with stronger staining in regions rich in fibers.
TS24: cerebral cortex	Present	Regionally restricted	2a_E16.5 Brain (mag) , 2a_E16.5 Brain	Expression is absent in a thin layer of cortical neurons.
TS24: telencephalon	Present	Regionally restricted	2a_E16.5 Brain (mag) , 2a_E16.5 Brain	Expression is absent around the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS24: peripheral nerve trunk	Present		2a_E16.5 Peripheral Nerve Trunk

Structure	Level	Pattern	Image	Note
TS26: lens	Present		2a_E18.5 Eye , 2a_E18.5 Eye (mag)
TS26: eyelid mesenchyme	Present	Regionally restricted	2a_E18.5 Eye (mag) , 2a_E18.5 Eye	Expression is in the sub-epithelial areas over the eyelids.
TS26: blood vessel	Present	Regionally restricted	2a_E18.5 Eye , 2a_E18.5 Eye (mag)	Expression is in retinal vessels.
TS26: retina	Present	Regionally restricted	2a_E18.5 Eye , 2a_E18.5 Eye (mag)	Expression is in retinal vessels.

Structure	Level	Pattern	Image	Note
TS26: brain	Present	Regionally restricted	2a_E18.5 Brain (mag) , 2a_E18.5 Brain	Expression is throughout the brain with stronger staining in regions rich in fibers.
TS26: cerebral cortex	Present	Regionally restricted	2a_E18.5 Brain (mag) , 2a_E18.5 Brain	Expression is absent in a thin layer of cortical neurons.
TS26: telencephalon	Present	Regionally restricted	2a_E18.5 Brain , 2a_E18.5 Brain (mag)	Expression is absent around the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present		2b_E12.5 Cartilage

Structure	Level	Pattern	Image	Note
TS21: dental lamina	Present		2b_E13.5 Tooth Germ , 2b_E13.5 Tooth Germ (mag)

Structure	Level	Pattern	Image	Note
TS21: cartilage	Present		2b_E13.5 Cartilage

Structure	Level	Pattern	Image	Note
TS21: bone	Present	Regionally restricted	2b_E13.5 Bone	Expression is in periosteal osteoblasts.

Structure	Level	Pattern	Image	Note
TS21: ganglion	Present		2b_E13.5 Ganglion

Structure	Level	Pattern	Image	Note
TS22: stellate reticulum	Weak		2b_E14.5 Tooth Germ (mag) , 2b_E14.5 Tooth Germ	Expression is in the region of the stellate reticulum.

Structure	Level	Pattern	Image	Note
TS22: cartilage	Present		2b_E14.5 Cartilage

Structure	Level	Pattern	Image	Note
TS22: bone	Present		2b_E14.5 Bone

Structure	Level	Pattern	Image	Note
TS22: ganglion	Present		2b_E14.5 Ganglion

Structure	Level	Pattern	Image	Note
TS23: dental lamina	Present		2b_E15.5 Tooth Germ (mag) , 2b_E15.5 Tooth Germ
TS23: enamel organ	Present	Regionally restricted	2b_E15.5 Tooth Germ (mag) , 2b_E15.5 Tooth Germ	Expression is in the inner enamel epithelium.

Structure	Level	Pattern	Image	Note
TS23: cartilage	Present		2b_E15.5 Cartilage

Structure	Level	Pattern	Image	Note
TS23: bone	Present		2b_E15.5 Bone

Structure	Level	Pattern	Image	Note
TS23: ganglion	Present		2b_E15.5 Ganglion

Structure	Level	Pattern	Image	Note
TS24: inner enamel epithelium	Present	Regionally restricted	2b_E16.5 Tooth Germ , 2b_E16.5 Tooth Germ (mag)
TS24: stratum intermedium	Present	Regionally restricted	2b_E16.5 Tooth Germ , 2b_E16.5 Tooth Germ (mag)
TS24: cervical loop	Weak		2b_E16.5 Tooth Germ (mag) , 2b_E16.5 Tooth Germ
TS24: stellate reticulum	Weak		2b_E16.5 Tooth Germ (mag) , 2b_E16.5 Tooth Germ
TS24: outer enamel epithelium	Absent		2b_E16.5 Tooth Germ (mag) , 2b_E16.5 Tooth Germ
TS24: jaw bone	Present	Regionally restricted	2b_E16.5 Tooth Germ , 2b_E16.5 Tooth Germ (mag)	Expression is in the alveolar bone trabeculae.

Structure	Level	Pattern	Image	Note
TS24: cartilage	Present		2b_E16.5 Cartilage

Structure	Level	Pattern	Image	Note
TS24: bone	Present		2b_E16.5 Bone

Structure	Level	Pattern	Image	Note
TS24: ganglion	Present		2b_E16.5 Ganglion

Structure	Level	Pattern	Image	Note
TS26: enamel organ	Very strong	Regionally restricted	2b_E18.5 Tooth Germ (mag) , 2b_E18.5 Tooth Germ	Expression is in the extracellular enamel matrix.
TS26: outer enamel epithelium	Very strong		2b_E18.5 Tooth Germ , 2b_E18.5 Tooth Germ (mag)
TS26: inner enamel epithelium	Very strong		2b_E18.5 Tooth Germ (mag) , 2b_E18.5 Tooth Germ	Expression is seen in ameloblasts.
TS26: stellate reticulum	Weak		2b_E18.5 Tooth Germ , 2b_E18.5 Tooth Germ (mag)
TS26: stratum intermedium	Weak		2b_E18.5 Tooth Germ , 2b_E18.5 Tooth Germ (mag)
TS26: dental papilla	Weak		2b_E18.5 Tooth Germ (mag) , 2b_E18.5 Tooth Germ	Expression is in odontoblasts and pre-dentin. Absent in dentin.

Structure	Level	Pattern	Image	Note
TS26: cartilage	Present		2b_E18.5 Cartilage

Structure	Level	Pattern	Image	Note
TS26: bone	Present		2b_E18.5 Bone

Structure	Level	Pattern	Image	Note
TS26: ganglion	Present		2b_E18.5 Ganglion

Structure	Level	Pattern	Image	Note
TS17: mesenchyme	Present
TS17: neural tube	Absent
TS17: peripheral nerve trunk	Absent

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted		Expression is in loosely packed mesenchymal cells at the base of the skull.
TS19: palatal shelf mesenchyme	Present
TS19: future spinal cord	Absent
TS19: surface ectoderm	Absent
TS19: trigeminal ganglion	Absent

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted		Expression is in fiber-like structures in the ventral spinal cord.
TS20: blood vessel	Present	Regionally restricted		Expression is in blood vessels surrounding the developing cartilage in the cranio-facial region.
TS20: palatal shelf mesenchyme	Present
TS20: bone	Absent
TS20: dermis	Absent
TS20: ganglion	Absent
TS20: skin	Absent

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present
TS21: palatal shelf mesenchyme	Present
TS21: vertebral cartilage condensation	Present	Regionally restricted		Expression is in hypertrophic chondrocytes.
TS21: connective tissue	Present	Regionally restricted		Expression is in the connective tissue surrounding the brain.
TS21: dermis	Absent
TS21: skin	Absent

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present
TS22: spinal cord	Present
TS22: trigeminal ganglion	Present
TS22: cranial ganglion	Present	Regionally restricted		Expression is in ganglia at the vicinity of the tooth germ.
TS22: cranium	Present	Regionally restricted		Expression is in osteoblasts, prehypertrophic and hypertrophic chondrocytes and in the cartilage beneath the perichondrium.
TS22: mandible	Present	Regionally restricted		Expression is in the mandibular alveolar bone and is faint in the endochondral mandibular condyle.
TS22: dermis	Absent
TS22: skin	Absent

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Strong
TS23: palatal shelf mesenchyme	Present
TS23: salivary gland	Present
TS23: trigeminal ganglion	Present
TS23: cranial ganglion	Present	Regionally restricted		Expression is in ganglia at the vicinity of the tooth germ.
TS23: cranium	Present	Regionally restricted		Expression is in hypertrophic chondrocytes and in the perichondrium.
TS23: blood vessel	Present	Regionally restricted		Expression is in blood vessels in various cranio-facial regions.
TS23: mandible	Present	Regionally restricted		Expression is in the mandibular alveolar bone and is faint in the endochondral mandibular condyle.
TS23: dermis	Absent
TS23: skin	Absent

Structure	Level	Pattern	Image	Note
TS24: oral epithelium	Present
TS24: trigeminal ganglion	Present
TS24: cranial ganglion	Present	Regionally restricted		Expression is in ganglia at the vicinity of the tooth germ.
TS24: cranium	Present	Regionally restricted		Expression is in hypertrophic chondrocytes and in the perichondrium.
TS24: blood vessel	Present	Regionally restricted		Expression is in blood vessels in various cranio-facial regions.
TS24: mandible	Present	Regionally restricted		Expression is in endochondral and membranous bone.
TS24: maxilla	Present	Regionally restricted		Expression is in endochondral and membranous bone.
TS24: jaw bone	Present	Regionally restricted		Expression is in the periosteum.
TS24: dermis	Absent
TS24: skin	Absent

Structure	Level	Pattern	Image	Note
TS26: trigeminal ganglion	Present
TS26: cranial ganglion	Present	Regionally restricted		Expression is in ganglia at the vicinity of the tooth germ.
TS26: cranium	Present	Regionally restricted		Expression is in chondrocytes.
TS26: blood vessel	Present	Regionally restricted		Expression is in blood vessels in the hypertrophic cartilage.
TS26: mandible	Present	Regionally restricted		Expression is in endochondral and membranous bone.
TS26: maxilla	Present	Regionally restricted		Expression is in endochondral and membranous bone.
TS26: jaw bone	Present	Regionally restricted		Expression is in the periosteum.
TS26: dermis	Weak
TS26: epidermis	Absent
TS26: salivary gland	Absent