Antibody:	anti-FAM20C
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: bone	Absent			No expression in any chondrogenic or osteogenic tissue at E13.5
TS21: cartilage	Absent			No expression in any chondrogenic or osteogenic tissue at E13.5

Structure	Level	Pattern	Image	Note
TS22: bone	Absent			No expression in long bones at E14.5.

Structure	Level	Pattern	Image	Note
TS23: femur	Present	Regionally restricted	2B	Expression in primary ossification center (POC).

Structure	Level	Pattern	Image	Note
TS23: bone	Present	Regionally restricted	2D	Expression in osteoblasts and extracellular matrix.

Structure	Level	Pattern	Image	Note
TS24: femur	Present	Regionally restricted	2F	Expression in primary ossification center (POC). Authors report relatively increased levels and broader areas of expression in osseus tissue compared with its distribution at E15.5.

Structure	Level	Pattern	Image	Note
TS24: femur	Present	Regionally restricted	2H	Expression in osteoblasts and osteocytes.

Structure	Level	Pattern	Image	Note
TS27: femur	Present	Regionally restricted		No expression in the primary ossification center (POC).

Structure	Level	Pattern	Image	Note
TS27: trabecular bone	Present	Regionally restricted	2J	Expression in cells of trabecular bone and cortical bone.

Structure	Level	Pattern	Image	Note
TS27: bone tissue	Present	Regionally restricted	2L	Expression in osteoblasts and osteocytes.

Structure	Level	Pattern	Image	Note
TS28: femur	Present	Regionally restricted	3B	No expression in the primary ossification center (POC).

Structure	Level	Pattern	Image	Note
TS28: bone tissue	Present	Regionally restricted	3D

Structure	Level	Pattern	Image	Note
TS28: femur	Strong	Regionally restricted	3H	Authors report maximal level of expression in femur at 3 weeks after birth.

Structure	Level	Pattern	Image	Note
TS28: bone tissue	Present	Regionally restricted	3I	Expression in articular chondrocytes and osteocytes. Authors report abundant expression in articular chondrocytes, proliferative chondrocytes, and prehypertrophic and hypertrophic chondrocytes.
TS28: cartilage	Present	Regionally restricted	3I	Expression in articular chondrocytes and osteocytes. Authors report abundant expression in articular chondrocytes, proliferative chondrocytes, and prehypertrophic and hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS28: trabecular bone	Present	Regionally restricted	3J	Expression in osteoblasts along the osteoid of trabecular bone and in osteocytes.

Structure	Level	Pattern	Image	Note
TS28: bone tissue	Present	Regionally restricted		Author report expression in mature osteocytes and in the extracellular matrix of the femur.

Structure	Level	Pattern	Image	Note
TS28: femur	Weak	Regionally restricted	3N	Expression level dramatically reduced when compare with earlier time points.

Structure	Level	Pattern	Image	Note
TS28: bone tissue	Present	Regionally restricted	3O	Expression in articular chondrocytes and osteocytes, and in the extracellular matrix of the bone.
TS28: cartilage	Present	Regionally restricted	3O	Expression in articular chondrocytes and osteocytes, and in the extracellular matrix of the bone.

Structure	Level	Pattern	Image	Note
TS28: trabecular bone	Present	Regionally restricted	3P	Expression in osteoblasts lining trabecular bone in the femur.

Structure	Level	Pattern	Image	Note
TS22: cranium bone primordium	Present	Regionally restricted	4B	Expression in osteoblasts at ossification sites in craniofacial membrane bones, overlapping with mineralized areas.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present	Regionally restricted	4F	In first lower molar at cap stage, expression in dental epithelium of enamel organ.
TS22: lower jaw molar enamel organ	Present	Regionally restricted	4F	In first lower molar at cap stage, expression in dental epithelium of enamel organ.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present	Regionally restricted	4G	In first lower molar at cap stage, expression in dental epithelium of enamel organ.
TS22: lower jaw molar	Present	Regionally restricted	4G	In first lower molar at cap stage, expression in dental epithelium of enamel organ.

Structure	Level	Pattern	Image	Note
TS22: lower jaw alveolar sulcus	Present	Regionally restricted	4H	Expression in osteoblasts of alveolar bone and extracellular matrix of osteoid.

Structure	Level	Pattern	Image	Note
TS23: cap stage tooth	Present	Regionally restricted		At late cap stage, expression in ameloblast layer and prospective odontoblast layer.
TS23: lower jaw molar dental papilla	Present	Regionally restricted		At late cap stage, expression in ameloblast layer and prospective odontoblast layer.
TS23: lower jaw molar enamel organ	Present	Regionally restricted		At late cap stage, expression in ameloblast layer and prospective odontoblast layer.
TS23: cerebral cortex	Present
TS23: cranial ganglion	Present

Structure	Level	Pattern	Image	Note
TS24: bell stage tooth	Present	Regionally restricted		At bell stage, experssion in ameloblast layer and prospective odontoblast layer.
TS24: lower jaw molar dental papilla	Present	Regionally restricted		At bell stage, experssion in ameloblast layer and prospective odontoblast layer.
TS24: lower jaw molar enamel organ	Present	Regionally restricted		At bell stage, experssion in ameloblast layer and prospective odontoblast layer.
TS24: cerebral cortex	Present
TS24: cranial ganglion	Present

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Strong	Regionally restricted	5E	Expression level in first lower molar increased dramatically in newborn mice compared with earlier stages.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar dental papilla	Present	Regionally restricted	5F	Expression in odontoblasts and ameloblasts.
TS27: lower jaw molar enamel organ	Present	Regionally restricted	5F	Expression in odontoblasts and ameloblasts.
TS27: predentin	Present		5F

Structure	Level	Pattern	Image	Note
TS27: bone tissue	Present	Regionally restricted	5G	Expression in osteoblasts and osteocytes. Expression decreases with age.

Structure	Level	Pattern	Image	Note
TS28: lower jaw molar	Present	Regionally restricted	6C	Expression in first lower molar.

Structure	Level	Pattern	Image	Note
TS28: lower jaw alveolar sulcus	Present	Regionally restricted	6D	Expression in odontoblasts, ameloblasts, osteoblasts, and osteocytes.
TS28: lower jaw molar dental papilla	Present	Regionally restricted	6D	Expression in odontoblasts, ameloblasts, osteoblasts, and osteocytes.
TS28: lower jaw molar enamel organ	Present	Regionally restricted	6D	Expression in odontoblasts, ameloblasts, osteoblasts, and osteocytes.

Structure	Level	Pattern	Image	Note
TS28: lower jaw alveolar sulcus	Present	Regionally restricted	6G	Expression in first lower molar in first lower molar in odontoblasts and osteocytes.
TS28: lower jaw molar dental papilla	Present	Regionally restricted	6G	Expression in first lower molar in first lower molar in odontoblasts and osteocytes.

Structure	Level	Pattern	Image	Note
TS28: dental sac	Present	Regionally restricted	7B	Expression in first lower molar in odontoblasts and cementoblasts.
TS28: lower jaw molar dental papilla	Present	Regionally restricted	7B	Expression in first lower molar in odontoblasts and cementoblasts.

Structure	Level	Pattern	Image	Note
TS28: lower jaw incisor dental papilla	Present	Regionally restricted	7D	Expression in odontoblasts and ameloblasts.
TS28: lower jaw incisor enamel organ	Present	Regionally restricted	7D	Expression in odontoblasts and ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: lower jaw molar dental papilla	Present	Regionally restricted	7F	Expression in first lower molar. No expression in odontoblasts; Expression in a small portion of odntoblasts beneath the tooth cusp tips. Expression nearly faded away at 7 weeks.

Structure	Level	Pattern	Image	Note
TS28: lower jaw incisor dental papilla	Present	Regionally restricted	7H	Expression in odontoblasts, ameloblasts and periodontal ligament (PDL) matrix.
TS28: lower jaw incisor enamel organ	Present	Regionally restricted	7H	Expression in odontoblasts, ameloblasts and periodontal ligament (PDL) matrix.
TS28: periodontal ligament	Present	Regionally restricted	7H	Expression in odontoblasts, ameloblasts and periodontal ligament (PDL) matrix.