Probe:	Fam20c cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: bone	Absent			No expression in any chondrogenic or osteogenic tissue at E13.5.
TS21: cartilage	Absent			No expression in any chondrogenic or osteogenic tissue at E13.5.

Structure	Level	Pattern	Image	Note
TS22: bone	Absent			No expression in long bones at E14.5.

Structure	Level	Pattern	Image	Note
TS23: femur	Present	Regionally restricted	2A	Expression in primary ossification center (POC).

Structure	Level	Pattern	Image	Note
TS23: bone	Present	Regionally restricted	2C	Expression in osteoblasts and osteoprogenitor cells.

Structure	Level	Pattern	Image	Note
TS24: femur	Present	Regionally restricted	2E	Expression in primary ossification center (POC). Authors report relatively increased levels and broader areas of expression in osseus tissue compared with its distribution at E15.5.

Structure	Level	Pattern	Image	Note
TS24: bone	Present	Regionally restricted	2G	Expression in osteoblasts, osteocytes and osteoprogenitor cells.

Structure	Level	Pattern	Image	Note
TS27: femur	Present	Regionally restricted		No expression in the primary ossification center (POC).

Structure	Level	Pattern	Image	Note
TS27: trabecular bone	Present	Regionally restricted	2I	Expression in cells of trabecular bone and cortical bone.

Structure	Level	Pattern	Image	Note
TS27: bone tissue	Present	Regionally restricted	2K	Expression in osteooblasts and osteocytes.

Structure	Level	Pattern	Image	Note
TS28: femur	Present	Regionally restricted	3A

Structure	Level	Pattern	Image	Note
TS28: bone tissue	Present	Regionally restricted	3C	Expression in osteoblasts and immature osteocytes, but not in mature osteocytes.

Structure	Level	Pattern	Image	Note
TS28: femur	Strong	Regionally restricted	3E	Authors report maximal level of expression in femur at 3 weeks after birth.

Structure	Level	Pattern	Image	Note
TS28: cartilage	Present	Regionally restricted	3F	Expression in articular chondrocytes. Authors report abundant expression in articular chondrocytes, proliferative chondrocytes, and prehypertrophic and hypertrophic choncrocytes.

Structure	Level	Pattern	Image	Note
TS28: trabecular bone	Present	Regionally restricted	3G	Expression in osteoblasts along the osteoid of trabecular bone.

Structure	Level	Pattern	Image	Note
TS28: femur	Present	Regionally restricted	3K	Expression level dramatically reduced when compare with earlier time points.

Structure	Level	Pattern	Image	Note
TS28: bone tissue	Present	Regionally restricted	3L	Expression absent in articular chondrocytes and mature osteocytes.
TS28: cartilage	Present	Regionally restricted	3L	Expression absent in articular chondrocytes and mature osteocytes.

Structure	Level	Pattern	Image	Note
TS28: trabecular bone	Present	Regionally restricted	3M	Expression in osteoblasts lining trabecular bone in the femur.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present	Regionally restricted	4C	In first lower molar at cap stage, expression in dental epithelium of enamel organ.
TS22: lower jaw molar epithelium	Present	Regionally restricted	4C	In first lower molar at cap stage, expression in dental epithelium of enamel organ.

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present	Regionally restricted	4D	In first lower molar at cap stage, expression in dental epithelium of enamel organ.
TS22: lower jaw molar epithelium	Present	Regionally restricted	4D	In first lower molar at cap stage, expression in dental epithelium of enamel organ.

Structure	Level	Pattern	Image	Note
TS22: lower jaw alveolar sulcus	Present	Regionally restricted	4E	Expression in osteoblasts of alveolar bone.

Structure	Level	Pattern	Image	Note
TS23: cap stage tooth	Present	Regionally restricted		At late cap stage, expression in ameloblast layer and prospective odontoblast layer.
TS23: lower jaw molar dental papilla	Present	Regionally restricted		At late cap stage, expression in ameloblast layer and prospective odontoblast layer.
TS23: lower jaw molar enamel organ	Present	Regionally restricted		At late cap stage, expression in ameloblast layer and prospective odontoblast layer.
TS23: cerebral cortex	Present
TS23: cranial ganglion	Present

Structure	Level	Pattern	Image	Note
TS24: bell stage tooth	Present	Regionally restricted		At bell stage, expression in ameloblast layer and prospective odontoblast layer.
TS24: lower jaw molar dental papilla	Present	Regionally restricted		At bell stage, expression in ameloblast layer and prospective odontoblast layer.
TS24: lower jaw molar enamel organ	Present	Regionally restricted		At bell stage, expression in ameloblast layer and prospective odontoblast layer.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Strong	Regionally restricted	5A	Expression level in first lower molar increased dramatically in newborn mice compared with earlier stages.
TS27: cerebral cortex	Present		5A
TS27: cranial ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS27: lower jaw alveolar sulcus	Present	Regionally restricted	5B	Expression in first lower molar and alveolar bone.
TS27: lower jaw molar	Present	Regionally restricted	5B	Expression in first lower molar and alveolar bone.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar dental papilla	Present	Regionally restricted	5C	Expression in odontoblasts and ameloblasts.
TS27: lower jaw molar enamel organ	Present	Regionally restricted	5C	Expression in odontoblasts and ameloblasts.

Structure	Level	Pattern	Image	Note
TS27: lower jaw alveolar sulcus	Present	Regionally restricted	5D	Expression in osteoblasts but not in osteocytes.
TS27: lower jaw molar enamel organ	Present	Regionally restricted		Expression in dentin and enamel matrix.
TS27: predentin	Present	Regionally restricted		Expression in dentin and enamel matrix.

Structure	Level	Pattern	Image	Note
TS28: lower jaw molar	Present	Regionally restricted	6A	Expression in first lower molar.

Structure	Level	Pattern	Image	Note
TS28: lower jaw alveolar sulcus	Present	Regionally restricted	6B	Expression in odontoblasts, ameloblasts, and osteoblasts.
TS28: lower jaw molar dental papilla	Present	Regionally restricted	6B	Expression in odontoblasts, ameloblasts, and osteoblasts.
TS28: lower jaw molar enamel organ	Present	Regionally restricted	6B	Expression in odontoblasts, ameloblasts, and osteoblasts.

Structure	Level	Pattern	Image	Note
TS28: lower jaw molar dental papilla	Present	Regionally restricted	6E	Expression in first lower molar in odontoblasts. Expression decreases with age.
TS28: periodontal ligament	Present	Regionally restricted	6E	Expression in fibroblasts of periodontal ligament (PDL). Expression decreases with age.

Structure	Level	Pattern	Image	Note
TS28: dental sac	Present	Regionally restricted	7A	Expression in first lower molar in odontoblasts, cementoblasts, and fibroblasts of periodontal ligament (PDL).
TS28: lower jaw molar dental papilla	Present	Regionally restricted	7A	Expression in first lower molar in odontoblasts, cementoblasts, and fibroblasts of periodontal ligament (PDL).
TS28: periodontal ligament	Present	Regionally restricted	7A	Expression in first lower molar in odontoblasts, cementoblasts, and fibroblasts of periodontal ligament (PDL).

Structure	Level	Pattern	Image	Note
TS28: lower jaw incisor dental papilla	Present	Regionally restricted	7C	Expression at the middle of the incisor root in odontoblasts and ameloblasts.
TS28: lower jaw incisor enamel organ	Present	Regionally restricted	7C	Expression at the middle of the incisor root in odontoblasts and ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: periodontal ligament	Present	Regionally restricted	7E	Expression in first lower molar in periodontal ligament (PDL) fibroblasts. Expression nearly faded away at 7 weeks.
TS28: lower jaw molar dental papilla	Absent		7E	No expression in odontoblasts.

Structure	Level	Pattern	Image	Note
TS28: lower jaw incisor dental papilla	Present	Regionally restricted	7G	Expression in middle of incisor root in odontoblasts, ameloblasts and periodontal ligament (PDL) fibroblasts.
TS28: lower jaw incisor enamel organ	Present	Regionally restricted	7G	Expression in middle of incisor root in odontoblasts, ameloblasts and periodontal ligament (PDL) fibroblasts.
TS28: periodontal ligament	Present	Regionally restricted	7G	Expression in middle of incisor root in odontoblasts, ameloblasts and periodontal ligament (PDL) fibroblasts.