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Gene Expression Data
Assay Details
Assay
Reference: J:124786 Xie M, et al., Functional implication of nucleolin in the mouse first molar development. J Biol Chem. 2007 Aug 10;282(32):23275-83
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5902040
Gene symbol: Ncl
Gene name: nucleolin
Antibody: C23 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 2B E12.0: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: mandible primordium Present Regionally restricted 2B E12.0 Authors report protein expression pattern similar to that of mRNA at early bud stage. Expression in thickened oral/dental epithelium and in underlying dental mesenchyme of lower first molar.
Specimen 2B E14.0: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: lower jaw molar epithelium Present Regionally restricted 2B E14.0 Expression mainly in epithelial cells of tooth bud.
TS22: tooth bud Present Regionally restricted 2B E14.0 Expression mainly in epithelial cells of tooth bud.
TS22: lower jaw molar dental papilla Strong 2B E14.0 Authors state that protein expression in dental papilla appeared to be stronger than that of mRNA.
Specimen 2B E15.0: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cap stage tooth Present Regionally restricted 2B E15.0
TS23: lower jaw molar enamel organ Present Regionally restricted 2B E15.0 Expression in whole enamel organ, including primary enamel knot (PEK).
TS23: enamel knot Present 2B E15.0
Specimen 2C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cap stage tooth Present Regionally restricted 2C
TS23: lower jaw molar enamel organ Strong Regionally restricted 2C Strong protein expression in primary enamel knot (PEK). Dot-like appearance of expression in cytoplasm and nucleus.
Specimen 2B E16.0: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: early bell stage tooth Present Regionally restricted 2B E16.0
TS24: lower jaw molar enamel organ Strong Regionally restricted 2B E16.0 Strong expression in inner enamel epitheium, cervical loop, and part of outer enamel epithelial cells.
TS24: cervical loop Present 2B E16.0
TS24: lower jaw molar dental papilla Present 2B E16.0
Specimen 2B E17.0: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: mid bell stage tooth Present Regionally restricted 2B E17.0
TS25: lower jaw molar enamel organ Present Regionally restricted 2B E17.0 Expression restricted to inner enamel epithelial cells.
Specimen 2B E18.0: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: late bell stage tooth Present Regionally restricted 2B E18.0 Authors report protein expression pattern simlar to that of mRNA at bell stage.
TS26: lower jaw molar enamel organ Present Regionally restricted 2B E18.0 Expression restricted to inner enamel epithelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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