Antibody:	STC antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Present	Regionally restricted	1C	Expression in both rostral and and caudal zones of each sclerotomal condensation.
TS19: dorsal root ganglion	Strong		1C

Structure	Level	Pattern	Image	Note
TS21: thoracic vertebral cartilage condensation	Present	Regionally restricted	1D inset	Expression in perichondrium and chondrocytes of thoracic vertebral cartilage elements
TS21: intervertebral disc	Strong		1D inset

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Present		1F	Co-localization with mRNA expression in cells of intervertebral disc regions between vertebral bodies.

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Present		1G	Expression also evident in pre-chondrocytes of vertebral bodies.
TS21: thoracic vertebral cartilage condensation	Weak	Regionally restricted	1G	Low levels of expression in regions adjacent to intervertebral discs, destined to become coccygeal vertebrae.

Structure	Level	Pattern	Image	Note
TS22: intervertebral disc	Present
TS22: tail vertebral pre-cartilage condensation	Weak	Regionally restricted		Low levels of expression in regions adjacent to intervertebral discs, destined to become coccygeal vertebrae.

Structure	Level	Pattern	Image	Note
TS23: C1 vertebra	Present	Regionally restricted	1I	Co-localization with protein expression in cells bordering the vertebral elements and cells within cartilage primordium of vertebrae.
TS23: C2 vertebra	Present	Regionally restricted	1I	Co-localization with protein expression in cells bordering the vertebral elements and cells within cartilage primordium of vertebrae.

Structure	Level	Pattern	Image	Note
TS23: C2 vertebra	Present	Regionally restricted	1J	High expression in chondrocytes with dispersed chromatin. No expression in chondrocytes with condensed chromatin.

Structure	Level	Pattern	Image	Note
TS15: limb bud	Weak			Low levels of expression first seen in limb buds at E9.5.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present	Regionally restricted		Expression clearly evident in cells bordering proximal and distal regions of cartilaginous condensations within limbs.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present	Regionally restricted		Most of expression confined to mesenchyme cells immediately surrounding digital rays.

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit mesenchyme	Present	Regionally restricted	3C	Most of expression confined to mesenchyme cells immediately surrounding digital rays.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit 3 metacarpus pre-cartilage condensation	Weak	Regionally restricted	3F	Light expression in select chondrocytes of 3rd metacarpal.

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit	Strong	Regionally restricted	3J	Highest levels of expression in chondrocytes in tarsal and digit cartilaginous elements.
TS23: tarsal bone	Strong	Regionally restricted	3J	Highest levels of expression in chondrocytes in tarsal and digit cartilaginous elements.
TS23: foot joint	Weak	Regionally restricted	3J	Least expression in regions expressing perichondrial and interzone cells.
TS23: foot skin	Strong		3J

Structure	Level	Pattern	Image	Note
TS24: foot joint	Weak	Regionally restricted	3L	Weak expression in interzone cells and perichondrocytes.
TS24: foot mesenchyme	Weak	Regionally restricted	3L	Weak expression in interzone cells and perichondrocytes.
TS24: tarsal bone	Present	Regionally restricted	3L	Most expression confined to underlying chondrocytes of tarsal elements.

Structure	Level	Pattern	Image	Note
TS22: shoulder joint primordium	Present	Regionally restricted	4B	Expression in shoulder joint interzone cells in both perichondrocytes and chondrocytes of underlying hyaline cartilage.

Structure	Level	Pattern	Image	Note
TS26: forelimb digit 2 interphalangeal joint	Present	Regionally restricted	4E	Expression in underlying hyaline cartilage cells on proximal and distal articular surfaces. Expression in underlying chondrocytes and associated ligaments.

Structure	Level	Pattern	Image	Note
TS25: femur	Present	Regionally restricted	4G , 4F , 4H

Structure	Level	Pattern	Image	Note
TS25: femur	Present	Regionally restricted	4I-3	Expression co-localized with mRNA to perichondrocytes on articular surfaces of femur. Expression in chondrocytes of underlying resting zone.

Structure	Level	Pattern	Image	Note
TS25: femur	Present	Regionally restricted	4J	Expression in growth plate chondrocytes of proliferative and prehypertrophic zones.

Structure	Level	Pattern	Image	Note
TS25: femur	Weak	Regionally restricted	4K	Weakest expression in prehypertrophic and hypertrophic chondrocytes of the growth plate.

Structure	Level	Pattern	Image	Note
TS25: femur	Strong	Regionally restricted	4L	Highest levels of expression in osteoblasts of trabecular zone. Weak expression in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS25: femur	Present	Regionally restricted	4M	Expression in perichondrocytes and underlying resting chondrocytes in proximal femur.