Probe:	STC probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: axial skeleton	Present			Authors report expression in axial skeleton beginning at E10.5.

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Present	Regionally restricted	1A	Expression restricted to rostral, less condensed halves of a dozen or more individual sclerotomal segments along the post-cranial axis.

Structure	Level	Pattern	Image	Note
TS19: sclerotome	Present	Regionally restricted	1B	Little or no expression in caudal half of sclerotome, characterized by greater cell density.

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Strong		1D	Intense expression in sacral intervertebral discs between precartilage vertebral bodies. No expression in thoracic intervertebral disc.
TS21: rib cartilage condensation	Absent		1D	No expression in rostral, more developmenetally advanced thoracic vertebrae and ribs, defined by a perichondrium.
TS21: thoracic vertebral cartilage condensation	Absent		1D	No expression in rostral, more developmenetally advanced thoracic vertebrae and ribs, defined by a perichondrium.

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Present	Regionally restricted	1E	Co-localization with protein expression in cells of intervertebral disc regions between precartilage vertebral bodies.

Structure	Level	Pattern	Image	Note
TS21: tail somite	Absent			No expression in more caudal and less developmentally advanced coccygeal somites.

Structure	Level	Pattern	Image	Note
TS22: intervertebral disc	Present	Regionally restricted		Greatest expression in intercoccygeal discs. Little, if any, expression in discs rostral to tail.

Structure	Level	Pattern	Image	Note
TS23: intervertebral disc	Weak			With completion of tail somite differentiation, only low levels of experssion in intervertebral disc mesenchyme cells.

Structure	Level	Pattern	Image	Note
TS23: C1 vertebra	Weak	Regionally restricted	1H	Expression confined to perichondrium of vertebral elements. Low levels of expression in cells bordering cartilage primordium of atlas C1 vertebra and axis C2 vertebra.
TS23: C2 vertebra	Weak	Regionally restricted	1H	Expression confined to perichondrium of vertebral elements. Low levels of expression in cells bordering cartilage primordium of atlas C1 vertebra and axis C2 vertebra.
TS23: basioccipital bone	Weak	Regionally restricted	1H	Low levels of expression in cells bordering cartilage primordium of basioccipital bone.

Structure	Level	Pattern	Image	Note
TS15: limb bud	Weak			Low levels of expression first seen in limb buds at E9.5.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Strong	Regionally restricted	3A , 3B	Areas of high expression proximal and distal to developing hindlimb precartilaginous elements
TS20: hindlimb digit mesenchyme	Weak	Regionally restricted	3A , 3B	Low levels of expression in mesenchyme cells surrounding the developing digital rays.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit 2 metacarpus pre-cartilage condensation	Strong	Regionally restricted	3D , 3E	Highest level of expression in most distal mesenchymal cell condensations of 2nd, 3rd and 4th metacarpal elements, coinciding with development of metacarpal-phalangeal joints.
TS21: forelimb digit 3 metacarpus pre-cartilage condensation	Strong	Regionally restricted	3D , 3E	Highest level of expression in most distal mesenchymal cell condensations of 2nd, 3rd and 4th metacarpal elements, coinciding with development of metacarpal-phalangeal joints.
TS21: forelimb digit 4 metacarpus pre-cartilage condensation	Strong	Regionally restricted	3D , 3E	Highest level of expression in most distal mesenchymal cell condensations of 2nd, 3rd and 4th metacarpal elements, coinciding with development of metacarpal-phalangeal joints.
TS21: metacarpus pre-cartilage condensation	Present	Regionally restricted	3E , 3D	No expression in chondroblasts of metacarpal elements.
TS21: carpus pre-cartilage condensation	Moderate	Regionally restricted	3E , 3D	Moderate level of expression between more proximal carpal elements. No expression in chondroblasts of carpal elements.

Structure	Level	Pattern	Image	Note
TS23: hindlimb digit 2 metatarsal-phalangeal joint	Strong	Regionally restricted	3G	High levels of expression in metatarsal-phalangeal interzone regions of 2nd, 3rd and 4th digits.
TS23: hindlimb interphalangeal joint	Present	Regionally restricted	3G	Expression at distal end of phalanx in putative interphalangeal joint interzone.
TS23: foot joint	Present	Regionally restricted	3G	Moderate expression in cells lining spaces between various tarsal elements. High level of expression in cavitating interzone between talus and calcaneus.

Structure	Level	Pattern	Image	Note
TS23: hindlimb interphalangeal joint	Present	Regionally restricted	3I , 3H	Greatest expression in metatarsal-phalangeal joint and interphalangeal joint regions.
TS23: metatarsal-phalangeal joint	Present	Regionally restricted	3I , 3H	Greatest expression in metatarsal-phalangeal joint and interphalangeal joint regions.

Structure	Level	Pattern	Image	Note
TS24: foot joint	Present	Regionally restricted	3K	Expression restricted to tarsal and metatarsal interzone regions.
TS24: foot mesenchyme	Present	Regionally restricted	3K	Moderate expression in ventral mesenchyme of hindlimb. Expression in perichondrocytes extending back from joint interzones on lateral margins of cartilage elements.

Structure	Level	Pattern	Image	Note
TS23: hindlimb joint	Present	Regionally restricted	3M	Expression in cavitating knee-joint region between femur and tibia.

Structure	Level	Pattern	Image	Note
TS22: shoulder joint primordium	Strong	Regionally restricted	4A	High levels of expression in shoulder joint interzone regions - future articular surfaces of humerus-scapula (gleno-humeral) joint.

Structure	Level	Pattern	Image	Note
TS26: forelimb interphalangeal joint	Present	Regionally restricted	4C	Expression in cavitating interphalangeal joint regions of forelimb, restricted to outermost layer of cells forming opposing articular surfaces.

Structure	Level	Pattern	Image	Note
TS26: forelimb digit 2 interphalangeal joint	Present	Regionally restricted	4D	Expression in 2nd interphalangeal cavitating interzone of forelimb digit.
TS26: forelimb digit 2 phalanx	Present	Regionally restricted	4D	Expression on distal articular surfaces of proximal phalanx and on opposing proximal edge of more distal (medial) phalanx.

Structure	Level	Pattern	Image	Note
TS25: femur	Present	Regionally restricted	4I-2 , 4I-1	Expression restricted to thin layer of perichondrocytes on articular surfaces of femur.