Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Gcgtm1.1(icre)Gkg
Name:	glucagon; targeted mutation 1.1, George K Gittes
MGI ID:	MGI:5898434
Synonyms:	GcgiCre
Gene:	Gcg  Location: Chr2:62304874-62313994 bp, - strand  Genetic Position: Chr2, 35.85 cM
Alliance:	Gcgtm1.1(icre)Gkg page

Germline Transmission:	Earliest citation of germline transmission: J:256199
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Gcgtm1.1(icre)Gkg expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Gcg (MGI:95674)   Mutation details: The targeting construct is designed to insert a codon improved Cre recombinase gene, followed by an FRT-flanked neomycin resistance (neo) cassette, into exon 2 of the gene. Unexpectedly the targeting process resulted in a duplication of all exons of Gcg gene, which spans 9 kb, and extends upstream for at least 17 kb and downstream for at least 7.8 kb, including the neighboring fibroblast activation protein (Fap) gene. Flp-mediated recombination removed the FRT-flanked neo cassette. Mice express Cre in 93-95% of alpha cells and in a negligible amount of beta cells. Cre expression is also seen in intestinal L cells and some neurons in brain. (J:256199)

Activity:	Tissue activity of this recombinase allele
Driver:	Gcg (mouse) Summary of all recombinase alleles driven by Gcg. Alliance mouse Gcg gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gcg Mutation:	32 strains or lines available

Original:	J:256199 Shiota C, et al., Gcg (CreERT2) knockin mice as a tool for genetic manipulation in pancreatic alpha cells. Diabetologia. 2017 Dec;60(12):2399-2408
All:	4 reference(s)