Probe:	Coup-TF cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The probe detected predominantly Nr2f1 and to a lesser extent Nr2f2.

Structure	Level	Pattern	Image	Note
TS16: future brain	Present	Regionally restricted	5A	Expressed in the neuroepithelium.
TS16: future spinal cord	Present	Regionally restricted	5A	Expressed in the differentiating motoneurons. Lower expression in the neuroepithelium.
TS16: limb	Present	Regionally restricted	5A	Expressed in the proximal part, extending more dorsally than ventrally.
TS16: 1st branchial arch mandibular component	Present		5A
TS16: dermatome	Present		5A
TS16: optic cup	Present		5A
TS16: otocyst	Present		5A
TS16: urogenital ridge	Present		5A

Structure	Level	Pattern	Image	Note
TS16: optic cup	Present	Regionally restricted	5B	Expressed in the area where the epithelium of the outer layer of the retina invaginates to form the inner layer.
TS16: latero-nasal process	Strong		5B
TS16: medial-nasal process	Absent		5B

Structure	Level	Pattern	Image	Note
TS16: future brain	Present	Regionally restricted	5C	Expressed in the neuroepithelium.
TS16: future spinal cord	Present	Regionally restricted	5C	Expressed in the differentiating motoneurons.
TS16: dermatome	Present		5C

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Strong	Regionally restricted	6A	Expressed in the neuroepitheliacl omponent of the different brain vesicles, in proximity of the ventricles.
TS20: fronto-nasal process mesenchyme	Strong		6A
TS20: neocortex	Strong		6A
TS20: cochlea epithelium	Present		6A
TS20: heart atrium	Present		6A
TS20: lung	Present		6A
TS20: medulla oblongata	Present		6A
TS20: mesonephros	Present		6A
TS20: metencephalon	Present		6A
TS20: midgut	Present		6A
TS20: pancreas	Present		6A
TS20: pericardium	Present		6A
TS20: choroid plexus	Present		6A	Moderately strong labeling.
TS20: spinal cord	Strong	Widespread	6A
TS20: trigeminal ganglion	Moderate		6A
TS20: trigeminal nerve	Moderate		6A
TS20: stomach	Present	Graded	6A	Expressed in a ventrodorsal gradient.
TS20: esophagus	Present	Regionally restricted	6A	Expressed in its abdominal part.
TS20: heart ventricle	Absent		6A
TS20: genital tubercle	Strong	Regionally restricted	6A	Expressed in the distal part.
TS20: liver	Trace		6A

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Widespread	6B
TS20: dorsal root ganglion	Very strong		6B
TS20: sclerotome	Absent		6B
TS20: stomach	Present	Graded	6B	Expressed in a ventrodorsal gradient.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		7A
TS22: cerebellum	Strong		7A	Relatively strong.
TS22: neocortex	Strong		7A	Relatively strong.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Strong		7B	Relatively strong.
TS22: neocortex	Strong		7B	Relatively strong.
TS22: choroid plexus	Weak		7B

Structure	Level	Pattern	Image	Note
TS22: retina	Weak	Regionally restricted	7C	Mostly in the outer nuclear layer.
TS22: lens	Absent		7C
TS22: pigmented retinal epithelium	Absent		7C
TS22: perioptic mesenchyme	Present	Regionally restricted	7C	Expressed in a layer of condensed mesenchyme surrounding the eye.

Structure	Level	Pattern	Image	Note
TS24: limb mesenchyme	Trace	Regionally restricted	7D	Very low expression in some areas of condensed mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: vibrissa follicle	Present	Regionally restricted	7E	Expressed in the dermal cells surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted		Expressed in the anterior part.
TS20: retina	Weak	Uniform
TS20: perioptic mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS22: liver	Absent
TS22: lung	Absent
TS22: small intestine	Absent
TS22: testis	Absent
TS22: adrenal gland	Weak
TS22: dorsal root ganglion	Weak
TS22: metanephros	Weak
TS22: male reproductive system	Weak	Regionally restricted		Expressed in mesonephritic tissues, which will give rise to the vas deferens and the epididymis.
TS22: limb mesenchyme	Weak	Regionally restricted		Expressed in some areas of condensed mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: liver	Absent