Gene Expression Data
Assay Details
Assay
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Reference:
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J:146496
Hagan JP, et al., At least ten genes define the imprinted Dlk1-Dio3 cluster on mouse chromosome 12qF1. PLoS One. 2009;4(2):e4352
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:5896432
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Gene symbol:
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Rtl1
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Gene name:
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retrotransposon Gaglike 1
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Probe:
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522Up/847Dn
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Visualized with:
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Ethidium bromide
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Assay notes:
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The RT-PCR products from this reaction were subjected to restriction digest to reveal allele-specific expression and imprinting. RT reactions were performed with either random hexamers or the strand-specific primers
for Peg11 477: TGGGTTCTGAGGCTTAGGGTGATAGAG (gene Rtl1 MGI:2656842) or Anti-Peg11 1052: GAAGACACTTGGAGAGCATCGTTCG (gene 6430411K18Rik MGI:1924130). Rtl1 was paternally expressed.
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Results
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Control |
Control |
Control |
Control |
Control |
Control |
Control |
P adult
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TS28: brain
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Present
|
Not Specified |
involves: 129S1/SvImJ * CZECHII/EiJ |
| Not Specified |
P adult
|
TS28: brain
|
Present
|
Not Specified |
involves: 129S1/SvImJ * CZECHII/EiJ |
| Not Specified |
Control |
P adult
|
TS28: brain
|
Present
|
Not Specified |
involves: 129S1/SvImJ * CZECHII/EiJ |
| Not Specified |
P adult
|
TS28: brain
|
Present
|
Not Specified |
involves: 129S1/SvImJ * CZECHII/EiJ |
| Not Specified |
Control |
P adult
|
TS28: brain
|
Present
|
Not Specified |
involves: 129S1/SvImJ * CZECHII/EiJ |
| Not Specified |
P adult
|
TS28: brain
|
Present
|
Not Specified |
involves: 129S1/SvImJ * CZECHII/EiJ |
| Not Specified |
|