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Gene Expression Data
Assay Details
Assay
Reference: J:19171 Holland PW, et al., In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization. J Cell Biol. 1987 Jul;105(1):473-82
Assay type: RNA in situ
MGI Accession ID: MGI:5888671
Gene symbol: Sparc
Gene name: secreted acidic cysteine rich glycoprotein
Probe: pC33.1
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: Probe size was reduced to an average size of 100 bases by limited alkaline hydrolysis. Sections were post-fixed with 4% paraformaldehyde.
Results
Specimen 1a/b: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: parietal endoderm Strong 1a/b
TS28: decidua Present 1a/b
TS13: trophoblast giant cell Weak 1a/b
Specimen 2a: postnatal adult (more )
Structure Level Pattern Image Note
TS28: adrenal cortex Strong 2a
TS28: adrenal gland zona fasciculata Strong Uniform 2a , 2c/d
TS28: adrenal medulla Absent 2a
TS28: adrenal gland zona glomerulosa Absent 2c/d , 2a
TS28: adrenal gland zona reticularis Absent 2c/d , 2a
Specimen 3a/b: postnatal week 2 (more )
Structure Level Pattern Image Note
TS28: ovary theca Strong Regionally restricted 3a/b Expression is in cells around the follicles.
TS28: oocyte Weak 3a/b
TS28: ovary follicle Weak 3a/b
Specimen 3e: postnatal adult (more )
Note: Pregnant female.
Structure Level Pattern Image Note
TS28: ovary theca Strong Regionally restricted 3f , 3e Expression is in the thecal layer of an antral follicle.
TS28: corpus luteum Strong 3f , 3e
Specimen 5a: postnatal week 2 (more )
Structure Level Pattern Image Note
TS28: interstitium of the testis Strong Regionally restricted 5a Expression is strongest in the cells between the seminiferous tubules.
TS28: testis Strong Regionally restricted 5a Expression is in the capsule.
TS28: seminiferous tubule Weak 5a
Specimen 5c/d: postnatal adult (more )
Structure Level Pattern Image Note
TS28: interstitium of the testis Strong 5c/d
TS28: seminiferous tubule Weak 5c/d
Specimen 6a/b: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: axial skeleton Strong 6a/b
TS27: cranium Strong 6a/b
TS27: jaw bone Strong 6a/b
TS27: stomach submucosa Strong 6a/b
TS27: tooth Strong 6a/b
TS27: vibrissa Strong 6a/b
TS27: peripheral nerve trunk Strong Regionally restricted 6a/b Expression is in the infraorbital nerve trunk.
TS27: thyroid gland Strong Regionally restricted 6a/b Expression is in the interstitial stroma.
TS27: hippocampus Present Regionally restricted 6a/b
TS27: telencephalon pia mater Present Regionally restricted 6a/b
Specimen 7a/b: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: rib Strong 7a/b Expression is strongest in cells on the periosteal and endosteal surfaces and is weak in cartilage.
Specimen 8a/b: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: jaw bone Strong Regionally restricted 8a/b Expression is stronger on the surface away from the teeth, where bone deposition is most active, than on the inner surface of the tooth cavity.
TS27: peripheral nerve trunk Present Regionally restricted 8a/b Expression is in the infraorbital nerve trunk.
Specimen 8c: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: molar Present 8c Expression is in the cytoplasm of the odontoblasts of the first molar.
TS27: predentin Present 8c Expression is in the cytoplasm of the odontoblasts of the first molar.
TS27: molar enamel organ Weak 8c
Specimen 8d: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: alveolar process Present 8d Expression is in the periosteal region, weak expression in mononucleated mature osteocytes within the bone.
Specimen 9a: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: snout Very strong Regionally restricted 9a , 9c/d Expression is in the connective tissue sheath around the whisker follicles.
Specimen 9b: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: dermis Present 9b
Specimen Table 1 E8.5: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: heart Strong
TS13: parietal endoderm Strong
TS28: decidua Strong
TS13: embryo endoderm Weak Regionally restricted Expression is in the visceral endoderm.
TS13: trophectoderm Weak
TS13: neural ectoderm Absent
TS13: paraxial mesenchyme Absent
Specimen Table 1 Newborn: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: dermis Strong
TS27: peripheral nerve trunk Strong
TS27: stomach submucosa Strong
TS27: bone Strong Regionally restricted Periosteal cells of membrane and endochondrial bone
TS27: predentin Strong Regionally restricted Expression is in odontoblasts.
TS27: thyroid gland Strong Regionally restricted Expression is in the intersttial stroma.
TS27: vibrissa follicle Strong Regionally restricted Expression is in the connective tissue sheath of whisker follicles.
TS27: cartilage Weak
TS27: heart Weak
TS27: lung Weak
TS27: incisor enamel organ Weak Regionally restricted Expression is in ameloblasts.
TS27: brain Present Regionally restricted Expression is absent in most neuronal cells of the brain.
TS27: hair follicle Present Regionally restricted Expression is absent in most most layers of the hair follicle.
TS27: epidermis Absent
TS27: liver Absent
TS27: stomach glandular epithelium Absent
Specimen Table 1 Adult: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: adrenal gland zona fasciculata Strong Female adrenal gland.
TS28: corpus luteum Strong
TS28: interstitium of the testis Strong
TS28: ovary follicle cell Strong
TS28: ovary theca Strong
TS28: Leydig cell Leydig cell Strong
TS28: testis Strong Regionally restricted Expression is in the capsule.
TS28: ovary follicle Present Regionally restricted Absent in preovulatory follicle cells.
TS28: adrenal gland zona glomerulosa Absent
TS28: adrenal gland zona reticularis Absent
TS28: adrenal medulla Absent
TS28: oocyte Absent
TS28: spermatogonium Absent
TS28: Sertoli cell Sertoli cell Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory