Probe:	p8P2a cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Fixative was 1% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS12: blood island	Present		2A
TS12: yolk sac endoderm	Present		2A
TS12: amnion	Absent		2A
TS12: embryo	Absent		2A
TS28: placenta	Absent		2A

Structure	Level	Pattern	Image	Note
TS13: trophectoderm	Present		2C

Structure	Level	Pattern	Image	Note
TS23: liver	Strong		3A
TS23: central nervous system	Present		3A
TS23: esophagus	Present		3A
TS23: heart	Present		3A
TS23: lung	Present		3A
TS23: vertebra	Strong	Regionally restricted	3A	Expression is in peripheral cells surrounding the endochondrial plates and in cells surrounding the endochondrial bone.
TS23: rib	Present	Regionally restricted	3A	Expression is in peripheral cells surrounding the endochondrial plates and in cells surrounding the endochondrial bone.
TS23: C1 vertebra	Present	Regionally restricted	3A	Expression is in cells surrounding the endochondrial bone.
TS23: pericardium	Absent		3A

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present		3C
TS23: clavicle	Present		3C
TS23: embryo mesenchyme	Strong	Regionally restricted	3C	Expression is in the mesenchyme surrounding the clavicle.
TS23: thyroid gland	Strong		3C
TS23: trachea	Strong		3C
TS23: thymus primordium	Strong	Regionally restricted	3C	Expression is in the lymphocytes.
TS23: submandibular gland	Absent		3C

Structure	Level	Pattern	Image	Note
TS23: bronchiole epithelium	Present	Regionally restricted	4A	Uniform labeling in epithelial cells, absent in some of the interstitial cells.
TS23: lung epithelium	Present	Regionally restricted	4A	Uniform labeling in epithelial cells, absent in some of the interstitial cells.

Structure	Level	Pattern	Image	Note
TS23: clavicle	Present	Regionally restricted	4C	Expression is in most cells.

Structure	Level	Pattern	Image	Note
TS23: liver parenchyma	Present		4D
TS23: hematopoietic system	Present	Regionally restricted	4D	Expression is in the hematopoietic precursors in the liver.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Present
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS19: nervous system	Present

Structure	Level	Pattern	Image	Note
TS21: liver	Present
TS21: lung	Present
TS21: nervous system	Present
TS21: bone	Present	Regionally restricted		Expression is in the endochondrial plates of the bone.

Structure	Level	Pattern	Image	Note
TS23: intestine	Absent
TS23: mammary gland primordium	Absent
TS23: metanephros	Absent
TS23: skin	Absent