Probe:	Gdd1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: embryo	Present			Expression first detectable at E9.5.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	1A GDD1-AS	Segmental bands of expression, progressively increasing in developing somites.

Structure	Level	Pattern	Image	Note
TS15: myotome	Present		1B GDD1 E9.5
TS15: dermatome	Absent		1B GDD1 E9.5
TS15: sclerotome	Absent		1B GDD1 E9.5

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		1B GDD1 E10.5

Structure	Level	Pattern	Image	Note
TS19: myotome	Present		1B GDD1 E11.5

Structure	Level	Pattern	Image	Note
TS20: myotome	Absent		1B GDD1 E12.5
TS20: perichondrium	Weak	Regionally restricted	1B GDD1 E12.5	Weak but significant level of expression detected in the perichondrium of the developing vertebral body.

Structure	Level	Pattern	Image	Note
TS21: perichondrium	Present	Regionally restricted	1B GDD1 E13.5	Expression in perichondrium of the developing vertebral body.

Structure	Level	Pattern	Image	Note
TS21: tongue	Present		1C GDD1 E13.5
TS21: vertebral cartilage condensation	Present	Regionally restricted	1C GDD1 E13.5	Expression in posterior ventromedial compartment of future vertebral bodies.
TS21: intervertebral disc	Strong	Widespread	1C GDD1 E13.5	Expression in intervertebral discs along the entire rostrocaudal axis, reaching maximal levels at E13.5.

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Present	Regionally restricted	1C GDD1 (middle panel)	Expression in intervertebral mesenchyme.
TS21: perichondrium	Strong	Regionally restricted	1C GDD1 (middle panel)	Expression in perichondrium of vertebral bodies.

Structure	Level	Pattern	Image	Note
TS22: forelimb muscle	Present		1D Fore-limb E14.5
TS22: sclerotome	Weak	Regionally restricted	1D Fore-limb E14.5	Expression in ventromedial sclerotomal cells gradually decreased.
TS22: forelimb digit mesenchyme	Present	Regionally restricted	1D Fore-limb E14.5	Expresssion in mesenchyme surrounding digits.
TS22: hand mesenchyme	Present	Regionally restricted	1D Fore-limb E14.5	Expresssion in mesenchyme surrounding carpal bones.
TS22: forelimb cartilage condensation	Absent		1D Fore-limb E14.5	Authors state that only background levels of expression were seen in developing limb bones.

Structure	Level	Pattern	Image	Note
TS22: hindlimb muscle	Present		1D Hind-limb E14.5
TS22: sclerotome	Weak	Regionally restricted	1D Hind-limb E14.5	Expression in ventromedial sclerotomal cells gradually decreased.
TS22: hindlimb digit mesenchyme	Present	Regionally restricted	1D Hind-limb E14.5	Expresssion in mesenchyme surrounding digits.
TS22: foot mesenchyme	Present	Regionally restricted	1D Hind-limb E14.5	Expresssion in mesenchyme surrounding tarsal bones.
TS22: hindlimb cartilage condensation	Absent		1D Hind-limb E14.5	Authors state that only background levels of expression were seen in developing limb bones.