Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:237216
Oetjen S, et al., Revisiting the neuronal localization and trafficking of CLN3 in juvenile neuronal ceroid lipofuscinosis. J Neurochem. 2016 Nov;139(3):456-470
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5881963
|
Gene symbol:
|
Cln3
|
Gene name:
|
CLN3 lysosomal/endosomal transmembrane protein, battenin
|
Probe:
|
Cln3 cDNA1
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
1a E12:
embryonic day 12.0
(more )
Specimen
1a E12:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1a E14:
embryonic day 14.0
(more )
Specimen
1a E14:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1a E16:
embryonic day 16.0
(more )
Specimen
1a E16:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1a E18:
embryonic day 18.0
(more )
Specimen
1a E18:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1b P3 (top):
postnatal day 3
(more )
Specimen
1b P3 (top):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: brain |
Weak |
Regionally restricted |
1b P3 (top)
|
Decreased expression levels in the brain.
|
TS27: hippocampus |
Present |
|
1b P3 (top)
|
|
Specimen
1b P3 (bottom):
postnatal day 3
(more )
Specimen
1b P3 (bottom):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebellum |
Present |
|
1b P3 (bottom)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
TS27: hippocampus |
Present |
|
1b P3 (bottom)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
Specimen
1b P7 (top):
postnatal day 7
(more )
Specimen
1b P7 (top):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
|
1b P7 (top)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
Specimen
1b P7 (bottom):
postnatal day 7
(more )
Specimen
1b P7 (bottom):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Weak |
Regionally restricted |
1b P7 (bottom)
|
Decreased expression levels in the brain.
|
Specimen
1b P21 (top):
postnatal day 21
(more )
Specimen
1b P21 (top):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 21
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
Regionally restricted |
1b P21 (top)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
Specimen
1b P21 (bottom):
postnatal day 21
(more )
Specimen
1b P21 (bottom):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 21
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
1b P21 (bottom)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
Specimen
1b adult (top left):
postnatal adult
(more )
Specimen
1b adult (top left):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
|
1b adult (top left)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
Specimen
1b adult (top right):
postnatal adult
(more )
Specimen
1b adult (top right):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
|
1b adult (top right)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
Specimen
1b adult (middle right):
postnatal adult
(more )
Specimen
1b adult (middle right):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Trace |
Regionally restricted |
1b adult (middle right)
|
Authors report strongly reduced expression; expression was hardly detectable.
|
Specimen
1b adult (bottom):
postnatal adult
(more )
Specimen
1b adult (bottom):
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
|
1b adult (bottom)
|
Relatively higher levels of expression only in areas of high cell density, such as the hippocampus and cerebellum.
|
|