Probe:	Chd2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: mesenchymal layer of pelvic urethra	Present		7S
TS22: urethra epithelium	Present		7S

Structure	Level	Pattern	Image	Note
TS25: intermediate cell layer of pelvic urethra of male	Strong		7T
TS25: basal cell layer of pelvic urethra of male	Weak		7T	Basal urethral epithelial staining is less apparent in male compared to female urethra.
TS25: mesenchymal layer of pelvic urethra of male	Weak		7T

Structure	Level	Pattern	Image	Note
TS28: lower urogenital tract of male	Ambiguous		7U

Structure	Level	Pattern	Image	Note
TS22: mesenchymal layer of pelvic urethra	Present		7V
TS22: urethra epithelium	Present		7V

Structure	Level	Pattern	Image	Note
TS25: basal cell layer of pelvic urethra of female	Present		7W
TS25: intermediate cell layer of pelvic urethra of female	Strong		7W
TS25: mesenchymal layer of pelvic urethra of female	Weak		7W

Structure	Level	Pattern	Image	Note
TS28: lower urogenital tract of female	Ambiguous		7X

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Moderate
TS22: pelvic urethra	Moderate	Regionally restricted		Medium expression was noted in the lamina propria, the muscularis mucosa, the submucosa, and the muscularis propria.
TS22: developing vasculature of pelvic urethra	Absent
TS22: mesenchyme derived from neural crest	Absent			No staining was detected in neural crest derivatives in the pelvic urethra.
TS22: epithelium of nephric duct of male	Moderate
TS22: mesenchyme of nephric duct of male	Moderate

Structure	Level	Pattern	Image	Note
TS22: epithelium of pelvic urethra	Moderate
TS22: pelvic urethra	Moderate	Regionally restricted		Medium expression was noted in the lamina propria, the muscularis mucosa, the submucosa, and the muscularis propria.
TS22: developing vasculature of pelvic urethra	Absent
TS22: mesenchyme derived from neural crest	Absent			No staining was detected in the neural crest derivatives in the pelvic urethra.
TS22: epithelium of paramesonephric duct of female	Moderate
TS22: mesenchyme of paramesonephric duct of female	Moderate

Structure	Level	Pattern	Image	Note
TS25: epithelium of pelvic urethra of male	Moderate
TS25: intermediate cell layer of pelvic urethra of male	Moderate
TS25: superficial cell layer of pelvic urethra of male	Weak
TS25: basal cell layer of pelvic urethra of male	Absent
TS25: lamina propria of pelvic urethra of male	Absent
TS25: muscle layer of pelvic urethra of male	Absent
TS25: muscularis mucosa of pelvic urethra of male	Absent
TS25: submucosa of pelvic urethra of male	Absent
TS25: mesenchymal pad of pelvic urethra of male	Absent
TS25: presumptive neural crest derivative of pelvic urethra of male	Absent
TS25: vasculature of pelvic urethra of male	Absent
TS25: epithelium of seminal vesicle	Moderate
TS25: muscle of seminal vesicle	Absent
TS25: vasculature of seminal vesicle	Absent
TS25: seminal vesicle	Moderate	Regionally restricted		Medium expression was detected in the epithelial layer, but no staining was detected in the mesenchymal layer or the adventitia.
TS25: epithelium of ejaculatory duct	Moderate
TS25: muscle of ejaculatory duct	Absent
TS25: vasculature of ejaculatory duct	Absent
TS25: ejaculatory duct	Moderate	Regionally restricted		Medium expression was detected in the epithelial layer, but no staining was detected in the mesenchymal layer.
TS25: prostate epithelium	Moderate
TS25: peri-prostatic mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS25: basal cell layer of pelvic urethra of female	Moderate
TS25: epithelium of pelvic urethra of female	Moderate
TS25: intermediate cell layer of pelvic urethra of female	Moderate
TS25: superficial cell layer of pelvic urethra of female	Weak
TS25: lamina propria of pelvic urethra of female	Absent
TS25: muscle layer of pelvic urethra of female	Absent
TS25: muscularis mucosa of pelvic urethra of female	Absent
TS25: submucosa of pelvic urethra of female	Absent
TS25: mesenchymal pad of pelvic urethra of female	Absent
TS25: presumptive neural crest derivative of pelvic urethra of female	Absent
TS25: vasculature of pelvic urethra of female	Absent
TS25: epithelium of upper vagina	Moderate
TS25: adventitia of upper vagina	Absent
TS25: muscle of upper vagina	Absent
TS25: vasculature of upper vagina	Absent
TS25: upper vagina	Moderate	Regionally restricted		Medium expression was detected in the epithelial layer, but no staining was detected in the mesenchymal layer.
TS25: epithelium of sinovaginal bulb	Moderate
TS25: adventitia of sinovaginal bulb	Absent
TS25: muscle of sinovaginal bulb	Absent
TS25: vasculature of sinovaginal bulb	Absent
TS25: sinovaginal bulb	Moderate	Regionally restricted		Medium expression was detected in the epithelial layer, but no staining was detected in the mesenchymal layer of the lower vagina.

Structure	Level	Pattern	Image	Note
TS28: basal cell layer of pelvic urethra of male	Moderate
TS28: epithelium of pelvic urethra of male	Moderate
TS28: intermediate cell layer of pelvic urethra of male	Moderate
TS28: superficial cell layer of pelvic urethra of male	Moderate
TS28: lamina propria of pelvic urethra of male	Absent
TS28: muscle layer of pelvic urethra of male	Absent
TS28: muscularis mucosa of pelvic urethra of male	Absent
TS28: submucosa of pelvic urethra of male	Absent
TS28: vasculature of pelvic urethra of male	Absent
TS28: mesenchymal pad of pelvic urethra of male	Absent
TS28: presumptive neural crest derivative of pelvic urethra of male	Absent
TS28: epithelium of seminal vesicle	Moderate
TS28: muscle of seminal vesicle	Absent
TS28: vasculature of seminal vesicle	Absent
TS28: seminal vesicle	Moderate	Regionally restricted		Medium expression was detected in the epithelial layer, but no staining was detected in the mesenchymal layer or the adventitia.
TS28: epithelium of ejaculatory duct	Moderate
TS28: muscle of ejaculatory duct	Absent
TS28: vasculature of ejaculatory duct	Absent
TS28: ejaculatory duct	Moderate	Regionally restricted		Medium expression was detected in the epithelial layer, but no staining was detected in the mesenchymal layer.
TS28: peri-prostatic mesenchyme	Weak
TS28: prostate epithelium	Weak

Structure	Level	Pattern	Image	Note
TS28: basal cell layer of pelvic urethra of female	Moderate
TS28: epithelium of pelvic urethra of female	Moderate
TS28: intermediate cell layer of pelvic urethra of female	Moderate
TS28: superficial cell layer of pelvic urethra of female	Moderate
TS28: lamina propria of pelvic urethra of female	Absent
TS28: muscle layer of pelvic urethra of female	Absent
TS28: muscularis mucosa of pelvic urethra of female	Absent
TS28: submucosa of pelvic urethra of female	Absent
TS28: mesenchymal pad of pelvic urethra of female	Absent
TS28: presumptive neural crest derivative of pelvic urethra of female	Absent
TS28: vasculature of pelvic urethra of female	Absent
TS28: epithelium of vagina	Moderate
TS28: muscle of vagina	Weak
TS28: adventitia of vagina	Absent
TS28: vasculature of vagina	Absent
TS28: vagina	Moderate	Regionally restricted		Medium expression was detected in the epithelial layer, but no staining was detected in the mesenchymal layer of the upper vagina.