Probe:	Ism1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	3B	Expression is anterior.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	3C	Expression is anterior.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	3D	Expression is anterior.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	3E	Expression is anterior.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		3F
TS13: midbrain-hindbrain junction	Present		3F
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	3F	Expression is in the anterior presomitic mesoderm.
TS13: somite	Present	Regionally restricted	3F	Expression is absent in the last formed somite.
TS13: foregut diverticulum endoderm	Weak		3F

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		3G
TS13: midbrain-hindbrain junction	Present		3G
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	3G	Expression is in the anterior presomitic mesoderm.
TS13: somite	Present	Regionally restricted	3G	Expression is absent in the last formed somite.
TS13: foregut diverticulum endoderm	Weak		3G

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		3H
TS13: midbrain-hindbrain junction	Present		3H
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	3H	Expression is in the anterior presomitic mesoderm.
TS13: somite	Present	Regionally restricted	3H	Expression is absent in the last formed somite.
TS13: foregut diverticulum endoderm	Weak		3H

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		3I
TS13: midbrain-hindbrain junction	Present		3I
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	3I	Expression is in the anterior presomitic mesoderm.
TS13: somite	Present	Regionally restricted	3I	Expression is absent in the last formed somite.
TS13: foregut diverticulum endoderm	Present		3I

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		3J
TS15: otocyst	Present		3J
TS15: dermomyotome	Present	Graded	3J	The gradient is along the anterior-posterior axis with stronger expression in the more posterior regions.
TS15: neural tube	Present	Graded	3J	In the dorsal neural tube staining is stronger at more anterior levels.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		3K
TS15: otocyst	Present		3K
TS15: dermomyotome	Present	Graded	3K	The gradient is along the anterior-posterior axis with stronger expression in the more posterior regions.
TS15: neural tube	Present	Graded	3K	In the dorsal neural tube staining is stronger at more anterior levels.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		3L
TS15: otocyst	Present		3L
TS15: dermomyotome	Present	Graded	3L	The gradient is along the anterior-posterior axis with stronger expression in the more posterior regions.
TS15: neural tube	Present	Graded	3L	In the dorsal neural tube staining is stronger at more anterior levels.

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		3M
TS15: otocyst	Present		3M
TS15: dermomyotome	Present	Graded	3M	The gradient is along the anterior-posterior axis with stronger expression in the more posterior regions.
TS15: neural tube	Present	Graded	3M	In the dorsal neural tube staining is stronger at more anterior levels.