Antibody:	anti-CGRP (C8198)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Absent		1A
TS20: spinal cord dorsal horn	Absent		1A
TS20: spinal cord ventral horn	Absent		1A

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventral horn	Weak	Regionally restricted	1B	Weak immunolabeling was detected in the motor neurons of the ventral horn of the cervical spinal cord.
TS21: dorsal root ganglion	Absent		1B
TS21: spinal cord dorsal horn	Absent		1B

Structure	Level	Pattern	Image	Note
TS22: spinal cord white matter	Weak	Regionally restricted	1C	Weak immunolabeling was detected in the most dorsal part of the posterior funiculus.
TS22: spinal cord dorsal horn	Weak	Regionally restricted	1C	Weak immunolabeling was detected in the dorsolateral region of the dorsal horn.
TS22: spinal cord ventral horn	Present	Regionally restricted	1C	Some large motor neurons were stained in the lateral part of the ventral horn.
TS22: dorsal root ganglion	Present	Regionally restricted	1C	Some neurons were positive.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present	Regionally restricted	1D	Many neurons were heavily labeled, and positive axon fibers were markedly increased in number.
TS24: spinal cord intermediate grey	Moderate		1D
TS24: spinal nerve	Moderate	Regionally restricted	1D	Dorsal roots were moderately stained.
TS24: spinal cord white matter	Moderate	Regionally restricted	1D	The posterior funiculus was moderately stained.
TS24: spinal cord lamina	Present	Regionally restricted	1D	Immunopositive axon fibers were distributed in the laminae I and II of the dorsal horn.
TS24: spinal cord ventral horn	Present	Regionally restricted	1D	Immunopositive motor neurons in the ventral horn increased in number and were more densely stained than at E14.

Structure	Level	Pattern	Image	Note
TS26: spinal cord lamina	Present	Regionally restricted	1E	Sensory axons were densely stained and distributed in lamina I through V in the dorsal horn.
TS26: spinal cord ventral horn	Present	Regionally restricted	1E	Immunopositive motor neurons were localized at the ventro-medial, ventro-lateral, and dorso-lateral region of the ventral horn.
TS26: dorsal root ganglion	Present	Regionally restricted	1E	Many but not all neurons and their central and peripheral axons were heavily labeled.

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present	Regionally restricted	2A	Immunolabel was noted in the sensory neurons.
TS27: spinal cord lamina	Present	Regionally restricted	2A	Immunolabel was localized in the dorsal sensory fibers in the laminae I-V of the dorsal horn.
TS27: spinal cord ventral horn	Present	Regionally restricted	2A	Immunolabel was detected in the motor neurons.
TS27: spinal nerve	Present	Regionally restricted	2A	Immunolabel was localized in the dorsal roots.

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present		2B	Immunolabel was not changed from earlier stages.
TS28: spinal cord white matter	Present	Regionally restricted	2B	Immunolabel in the posterior funiculus was not changed from earlier stages.
TS28: spinal cord ventral horn	Weak	Regionally restricted	2B	Staining in the motor neurons was markedly decreased.

Structure	Level	Pattern	Image	Note
TS28: spinal nerve	Present	Regionally restricted	2C	Many immunopositive sensory fibers were detected in dorsal roots.
TS28: spinal cord white matter	Present	Regionally restricted	2C	The posterior funiculus was labeled.
TS28: spinal cord lamina	Present	Regionally restricted	2C	Immunolabel was detected in sensory fibers in lamina I-V of the dorsal horn.
TS28: spinal cord ventral horn	Weak	Regionally restricted	2C	Weak label was detected in the motor neurons.

Structure	Level	Pattern	Image	Note
TS28: spinal nerve	Present	Regionally restricted	2D	Many immunopositive sensory fibers were detected in dorsal roots.
TS28: spinal cord white matter	Present	Regionally restricted	2D	The posterior funiculus was labeled.
TS28: spinal cord lamina	Present	Regionally restricted	2D	Immunolabel was detected in sensory fibers in lamina I-V of the dorsal horn.
TS28: spinal cord ventral horn	Weak	Regionally restricted	2D	Weak label was detected in the motor neurons.

Structure	Level	Pattern	Image	Note
TS22: spinal cord white matter	Present	Regionally restricted	3A	A few positive axon bundles were detected between the gracile fasciculus and cuneate fasciculus.

Structure	Level	Pattern	Image	Note
TS24: cuneate fasciculus	Present	Regionally restricted	3B	Immunopositive fibers increased in number and were detected in the dorsal part of the cuneate fasciculus.
TS24: gracile fasciculus	Present		3B

Structure	Level	Pattern	Image	Note
TS26: cuneate fasciculus	Present	Regionally restricted	3C	Immunolabel markedly increased in density and intensity.
TS26: gracile fasciculus	Present	Regionally restricted	3C	Immunolabel markedly increased in density and intensity.

Structure	Level	Pattern	Image	Note
TS27: cuneate fasciculus	Present	Regionally restricted	3D	Immunolabel markedly increased in density and intensity.
TS27: gracile fasciculus	Present	Regionally restricted	3D	Immunolabel markedly increased in density and intensity.

Structure	Level	Pattern	Image	Note
TS22: spinal cord dorsal horn	Weak	Regionally restricted	3E	On the surface of the lateral part of the dorsal horn, few axons and their varicosities were weakly stained.
TS22: spinal cord white matter	Ambiguous	Regionally restricted	3E	Lissauer's tract was faintly stained or negative.

Structure	Level	Pattern	Image	Note
TS24: spinal cord lamina	Present	Regionally restricted	3F	Immunostained fibers increased in the dorsal horn distributed in laminae I and II, fewer in lamina III.
TS24: spinal cord white matter	Present	Regionally restricted	3F	Some fibers ran into the posterior funiculus from Lissauer's tract, and some fibers vertically passed through Lissauer's tract.

Structure	Level	Pattern	Image	Note
TS26: spinal cord dorsal horn	Present	Regionally restricted	3G	Immunolabel markedly increased in density in the dorsal horn.
TS26: spinal cord lamina	Present	Regionally restricted	3G	Many axons invaded into laminae III-V.
TS26: spinal cord white matter	Present	Regionally restricted	3G	Some fibers ran into the posterior funiculus from Lissauer's tract, and some fibers vertically passed through Lissauer's tract.

Structure	Level	Pattern	Image	Note
TS27: spinal cord dorsal horn	Present		3H	Immunolabel was not different from E18.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Weak		3I	Some neurons were weakly stained, but the axons were not clearly detected.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		3J	Many neurons were stained, and a few axons were detected.

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		3K	Axons and neurons were clearly stained.

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		3L	Axons and neurons were clearly stained.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventral horn	Weak	Regionally restricted	4A	Some motor neurons were weakly labeled in the ventral horn.
TS22: spinal nerve	Weak	Regionally restricted	4A	Immunopositive nerve fibers were faintly detected in the ventral root.

Structure	Level	Pattern	Image	Note
TS24: spinal cord ventral horn	Present	Regionally restricted	4B	Dense immunolabel was detected within perikarya and proximal dendrites of motor neurons. Axons were clearly stained in the ventral horn.
TS24: spinal nerve	Present	Regionally restricted	4B	Axons were clearly stained within ventral roots.

Structure	Level	Pattern	Image	Note
TS26: spinal cord lamina	Present	Regionally restricted	4C	Immunopositive motor neurons were noted in lamina IX.
TS26: spinal cord ventral horn	Present	Regionally restricted	4C	Large motor neurons and their dendrites were strongly stained. Small motor neurons were weakly stained. Axons were detected within the ventral horn.

Structure	Level	Pattern	Image	Note
TS26: spinal nerve	Present	Regionally restricted	4D	Axons were detected within the ventral roots. Thin axons with varicosities were clearly stained.

Structure	Level	Pattern	Image	Note
TS27: spinal cord ventral horn	Present	Regionally restricted	4E	Motor neurons and fibers were stained.

Structure	Level	Pattern	Image	Note
TS28: spinal cord ventral horn	Weak	Regionally restricted	4F	Immunolabeling of motor neurons and fibers markedly decreased in intensity.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Absent
TS21: spinal cord dorsal horn	Absent
TS21: spinal cord white matter	Absent			The posterior funiculus was negative.
TS21: spinal nerve	Absent			The ventral root was negative.
TS21: spinal cord ventral horn	Weak	Regionally restricted		Weak staining in motor neurons.

Structure	Level	Pattern	Image	Note
TS22: cuneate fasciculus	Weak	Regionally restricted		Weak expression in the marginal line of the dorsal part.
TS22: gracile fasciculus	Weak	Regionally restricted		Weak expression in the marginal line of the dorsal part.
TS22: dorsal root ganglion	Weak
TS22: spinal cord dorsal horn	Ambiguous	Regionally restricted		The table labeled this structure as negative for staining. However, a footnote indicated immunopositive fibers were detected in the dorsolateral region of the dorsal horn.
TS22: spinal cord white matter	Weak	Regionally restricted		Weak expression in Lissauer's tract.
TS22: spinal cord ventral horn	Moderate	Regionally restricted		Moderate staining in motor neurons.
TS22: spinal nerve	Weak	Regionally restricted		Weak expression in ventral root.

Structure	Level	Pattern	Image	Note
TS24: cuneate fasciculus	Moderate
TS24: gracile fasciculus	Moderate
TS24: spinal cord lamina	Moderate
TS24: dorsal root ganglion	Strong
TS24: spinal cord white matter	Moderate	Regionally restricted		Expression in Lissauer's tract.
TS24: spinal cord ventral horn	Strong	Regionally restricted		Strong staining in motor neurons.
TS24: spinal nerve	Strong	Regionally restricted		Strong expression in ventral root.

Structure	Level	Pattern	Image	Note
TS26: cuneate fasciculus	Strong
TS26: dorsal root ganglion	Strong
TS26: gracile fasciculus	Strong
TS26: spinal cord lamina	Strong
TS26: spinal cord white matter	Strong	Regionally restricted		Strong expression in Lissauer's tract.
TS26: spinal cord ventral horn	Strong	Regionally restricted		Strong expression in motor neurons.
TS26: spinal nerve	Strong	Regionally restricted		Strong expression in ventral root.

Structure	Level	Pattern	Image	Note
TS27: cuneate fasciculus	Strong
TS27: dorsal root ganglion	Strong
TS27: gracile fasciculus	Strong
TS27: spinal cord lamina	Strong
TS27: spinal cord white matter	Strong	Regionally restricted		Strong expression in Lissauer's tract.
TS27: spinal cord ventral horn	Strong	Regionally restricted		Strong expression in motor neurons.
TS27: spinal nerve	Strong	Regionally restricted		Strong expression in ventral root.

Structure	Level	Pattern	Image	Note
TS28: cuneate fasciculus	Strong
TS28: dorsal root ganglion	Strong
TS28: gracile fasciculus	Strong
TS28: spinal cord lamina	Strong
TS28: spinal cord white matter	Strong	Regionally restricted		Strong expression in Lissauer's tract.
TS28: spinal cord ventral horn	Moderate	Regionally restricted		Expression in motor neurons.
TS28: spinal nerve	Moderate	Regionally restricted		Expression in ventral root.
TS28: corticospinal tract	Absent

Structure	Level	Pattern	Image	Note
TS28: cuneate fasciculus	Strong
TS28: dorsal root ganglion	Strong
TS28: gracile fasciculus	Strong
TS28: spinal cord lamina	Strong
TS28: spinal cord white matter	Strong	Regionally restricted		Strong expression in Lissauer's tract
TS28: spinal cord ventral horn	Moderate	Regionally restricted		Expression in motor neurons.
TS28: spinal nerve	Moderate	Regionally restricted		Expression in ventral root.
TS28: corticospinal tract	Absent

Structure	Level	Pattern	Image	Note
TS28: cuneate fasciculus	Strong
TS28: dorsal root ganglion	Strong
TS28: gracile fasciculus	Strong
TS28: spinal cord lamina	Strong
TS28: spinal cord white matter	Strong	Regionally restricted		Strong expression in Lissauer's tract.
TS28: spinal cord ventral horn	Moderate	Regionally restricted		Expression in motor neurons
TS28: spinal nerve	Moderate	Regionally restricted		Expression in ventral root.
TS28: corticospinal tract	Absent

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		S1A	Axons and neurons were clearly stained.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present		S1B	Many axons and neurons were stained.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present		S1C	Many axons and neurons were stained.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present		S1D	Many axons and neurons were stained.

Structure	Level	Pattern	Image	Note
TS28: spinal nerve	Present	Regionally restricted	S2A	Label was localized in the ventral root.
TS28: dorsal root ganglion	Present		S2A

Structure	Level	Pattern	Image	Note
TS28: spinal nerve	Present	Regionally restricted	S2B	Label was localized in the ventral root.
TS28: dorsal root ganglion	Present		S2B