Probe:	pSP73-11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	1A	Expression was detected in the zona limitans intrathalamica and along the alar/basal boundary in the diencephalon.
TS19: midbrain-hindbrain junction	Present		1A
TS19: midbrain	Present	Regionally restricted	1A	Expression was detected in anterior mesencephalon, as well as in the basal plate region.
TS19: forebrain-midbrain boundary region	Present	Regionally restricted	1A	Expression was detected in dorsal portions of the pretectum.

Structure	Level	Pattern	Image	Note
TS19: brain roof plate	Strong		1B	Intense signals were observed along the dorsal midline or roof plate. These signals extended anteriorly into the epithalamic zone and posteriorly throughout the spinal cord.
TS19: neural tube roof plate	Strong		1B

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Strong	Regionally restricted	1C	Intense signal surrounding the telencephalon was derived from mesenchymal tissues.
TS19: diencephalon	Present	Regionally restricted	1C	Intense staining was confined to the innermost ventricular zone of the hypothalamus. Signal was also localized along the dorsal ridge of the middle thalamic sulcus.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Strong	Regionally restricted	1D	Intense signals on the pial surface of the metencephalon and its junction to the myelencephalon were derived from mesenchymal tissues.
TS19: midbrain-hindbrain junction	Present	Non-Uniform	1D	Expression was more intense at the ventricular side but also extended to the superficial subpial zone.

Structure	Level	Pattern	Image	Note
TS21: zona limitans intrathalamica	Present		1E
TS21: diencephalon	Present	Regionally restricted	1E	Expression was detected along the alar/basal boundary.

Structure	Level	Pattern	Image	Note
TS21: zona limitans intrathalamica	Present	Regionally restricted	1F	More signal accumulated in the superficial side of the neural tube, especially in the posterior portions.

Structure	Level	Pattern	Image	Note
TS21: zona limitans intrathalamica	Present	Regionally restricted	1G	More signal accumulated in the superficial side of the neural tube, especially in the posterior portions.
TS21: forebrain-midbrain boundary region	Present	Regionally restricted	1G	Expression occurred in a curved pattern in the superficial subpial zone of the pretectum. The ventral staining ended at a deeper position of the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS21: zona limitans intrathalamica	Present		1H	Staining signal in the zona limitans intrathalamica could be detected even from the pial side of sagittally cut half brains.
TS21: forebrain-midbrain boundary region	Present	Regionally restricted	1H	Staining signal in the pretectum could be detected even from the pial side of sagittally cut half brains.

Structure	Level	Pattern	Image	Note
TS21: zona limitans intrathalamica	Present	Regionally restricted	1I	Signal was confined to the superficial subpial zone.
TS21: forebrain-midbrain boundary region	Present	Regionally restricted	1I	Pretectal signal was noted in the superficial zone.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present		1J
TS22: zona limitans intrathalamica	Present		1J
TS22: dorsal thalamus	Present	Regionally restricted	1J	Expression was detected in the anterior thalamic zone.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present		1K
TS22: zona limitans intrathalamica	Present		1K
TS22: dorsal thalamus	Present	Patchy	1K	Expression was in the differentiating zone.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present	Non-Uniform	1L	At E14.5, expression in the thalamus was much expanded, exhibiting some regional heterogeneity in its intensity. Expression was not in the ventricular zone of the thalamus.

Structure	Level	Pattern	Image	Note
TS22: basal ganglia	Present		1M
TS22: caudate-putamen	Present		1M
TS22: corpus striatum	Present		1M
TS22: telencephalon septum	Present		1M

Structure	Level	Pattern	Image	Note
TS22: zona limitans intrathalamica	Present		1N
TS22: thalamus	Present	Non-Uniform	1N	At E14.5, expression in the thalamus was much expanded, exhibiting some regional heterogeneity in its intensity.

Structure	Level	Pattern	Image	Note
TS22: zona limitans intrathalamica	Present		1O
TS22: thalamus	Present	Non-Uniform	1O	At E14.5, expression in the thalamus was much expanded, exhibiting some regional heterogeneity in its intensity.

Structure	Level	Pattern	Image	Note
TS22: zona limitans intrathalamica	Present		1P
TS22: thalamus	Present	Non-Uniform	1P	At E14.5, expression in the thalamus was much expanded, exhibiting some regional heterogeneity in its intensity.

Structure	Level	Pattern	Image	Note
TS22: mammillary area	Present	Regionally restricted	1Q	Expression was detected in the mammillary nuclei.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present

Structure	Level	Pattern	Image	Note
TS24: hypothalamus ventricular layer	Present		2A
TS24: mammillary area	Present	Regionally restricted	2A	Expression was in mammillary body.
TS24: main olfactory bulb	Present	Regionally restricted	2A	A rostral, cap-like pattern was observed with the most intense staining in the proximal zone.
TS24: cerebellar cortex	Present	Regionally restricted	2A	Expression was detected in developing nuclei and the superficial cortical layer.
TS24: deep cerebellar nucleus	Present	Regionally restricted	2A	Expression was detected in developing nuclei and the superficial cortical layer.

Structure	Level	Pattern	Image	Note
TS24: trigeminal nerve	Strong		2B
TS24: mammillary area	Present	Regionally restricted	2B	Expression was in mammillary body.
TS24: olfactory cortex	Present	Regionally restricted	2B	Expression was in the olfactory tubercle.
TS24: main olfactory bulb	Present	Regionally restricted	2B	A rostral, cap-like pattern was observed with the most intense staining in the proximal zone.
TS24: cerebellar cortex	Present	Regionally restricted	2B	Expression was detected in developing nuclei and the superficial cortical layer.
TS24: deep cerebellar nucleus	Present	Regionally restricted	2B	Expression was detected in developing nuclei and the superficial cortical layer.

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	2C	A rostral, cap-like pattern was observed with the most intense staining in the proximal zone.
TS24: olfactory cortex	Present	Regionally restricted	2C	Expression was in the olfactory tubercle.
TS24: caudate-putamen	Present		2C

Structure	Level	Pattern	Image	Note
TS24: caudate-putamen	Present		2D

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	2E	Expression extended from its anteriormost part to posterior areas.
TS24: epithalamus	Present		2E
TS24: hypothalamus ventricular layer	Present		2E

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	2F	Expression extended from its anteriormost part to posterior areas.
TS24: hypothalamus ventricular layer	Present		2F
TS24: pretectum	Present		2F

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted	2G	Expression was detected in the oculomotor nucleus, red nucleus, and substantia nigra.

Structure	Level	Pattern	Image	Note
TS24: superior colliculus	Present	Regionally restricted	2H	Expression was detected in the superficial layer.
TS24: brainstem nucleus	Present	Regionally restricted	2H	Expression was detected in the trigeminal motor nucleus.

Structure	Level	Pattern	Image	Note
TS24: brainstem nucleus	Present	Regionally restricted	2I	Expression was detected in the facial motor nucleus and the prepositus hypoglossal nucleus.

Structure	Level	Pattern	Image	Note
TS24: cerebellar cortex	Present	Regionally restricted	2J	Expression was detected in developing nuclei and the superficial cortical layer.
TS24: deep cerebellar nucleus	Present	Regionally restricted	2J	Expression was detected in developing nuclei and the superficial cortical layer.
TS24: brainstem nucleus	Present	Regionally restricted	2J	Expression was detected in the prepositus hypoglossal nucleus and the inferior olive nucleus.

Structure	Level	Pattern	Image	Note
TS24: brainstem nucleus	Present	Regionally restricted	2K	Expression was detected in the inferior olive nucleus.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Strong	Regionally restricted	3A	Strong signal was detected in the hypothalamus, perhaps in the regions corresponding to Prosomeres 5 and 6.
TS20: diencephalon	Present	Regionally restricted	3A	Expression was detected at both the alar and basal domains, although it was most intense along the boundary between these two domains.
TS20: zona limitans intrathalamica	Present		3A

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3C	Expression was detected at both the alar and basal domains, although it was most intense along the boundary between these two domains. Signal entirely overlaps with the Cdh4 signal at the dorsal border of the ventral thalamus, except at the anteriormost position of the zona limitans intrathalamica.
TS20: zona limitans intrathalamica	Present		3C