Probe:	Otx1 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Uniform	4a
TS20: lateral ganglionic eminence	Present	Regionally restricted	4a	Expressed in the germinal layer of the most lateral portion of lateral ganglionic eminence.
TS20: nasal epithelium	Present		4a
TS20: oral epithelium	Present		4a
TS20: thyroglossal duct	Present		4a
TS20: face	Present	Regionally restricted	4a	Expressed in ectodermal regions in the snout.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Uniform	4b
TS20: telencephalon	Present	Regionally restricted	4b	Expressed in the olfactory bulb.
TS20: epithalamus	Present		4b
TS20: nasal epithelium	Present		4b
TS20: oral epithelium	Present		4b
TS20: thyroglossal duct	Present		4b
TS20: zona limitans intrathalamica	Present		4b
TS20: thalamus	Present	Regionally restricted	4b	Expressed in dorsal region.
TS20: hypothalamus	Present	Regionally restricted	4b	Expressed in mammillary region.
TS20: face	Present	Regionally restricted	4b	Expressed in ectodermal regions in the snout.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Uniform	4c
TS20: telencephalon	Present	Regionally restricted	4c	Expressed in the olfactory bulb.
TS20: midbrain	Present	Regionally restricted	4c	Expressed in mesencephalic regions of tectum and tegmentum, possibly at the level of presumptive dorsal periventricular bundle.
TS20: nasal epithelium	Present		4c

Structure	Level	Pattern	Image	Note
TS20: nasal cavity	Present		5a	Including external ducts.
TS20: neocortex	Present		5a
TS20: telencephalon	Present	Regionally restricted	5a	Expressed in hippocampal and parahippocampal archipallium and selected palaeopallial and septal localizations. Expressed in the superior basimedial region.
TS20: lateral ganglionic eminence	Present	Regionally restricted	5a	Expressed in the germinal layer of lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: nasal cavity	Present		5b
TS20: eye	Present		5b	Expressed in ocular regions.
TS20: thalamus	Present		5b
TS20: diencephalon	Present	Regionally restricted	5b	Expressed in the germinal layer.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	5c	Expressed in mesencephalic regions of tectum and tegmentum, possibly at the level of presumptive dorsal periventricular bundle.
TS20: metencephalon	Present	Regionally restricted	5c	Expressed in the anterior, the presumptive pontine region.
TS20: pharynx	Present		5c
TS20: hypothalamus	Present	Regionally restricted	5c	Expressed in the posterior, including the sulcus lateralis hypothalami posterioris.

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present		6a
TS20: diencephalon	Present	Regionally restricted	6a	Expressed in two layers of cells orthogonal to the frontal surfacelayers of cells orthogonal to the frontal surface, surrounding the zona limitans intrathalamica present at the boundary between dorsal and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present		6d
TS20: brain	Present	Regionally restricted	6d	Expressed in the fasciculus retroflexus, the precursor of habenulo-interpenduncular tract, stria medullaris, including the region surrounding the posterior commissure, primordium of mammillotegmental tract, fornix and sulcus lateralis hypothalami posterioris.
TS20: diencephalon	Present	Regionally restricted	6d	Expressed in the germinal layer, particularly at the level of the diencephalicus dorsalis and medius.
TS20: thalamus	Present	Regionally restricted	6d	Expressed in ependymal layers of epithalamus and dorsal thalamus and in orthogonal layers of cells immediately above and below the sulcus diencephalicus medius.

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present		6e
TS20: brain	Present	Regionally restricted	6e	Expressed in the fasciculus retroflexus, the precursor of habenulo-interpenduncular tract, stria medullaris, including the region surrounding the posterior commissure, primordium of mammillotegmental tract, fornix and sulcus lateralis hypothalami posterioris.
TS20: diencephalon	Present	Regionally restricted	6e	Expressed in the germinal layer, particularly at the level of the diencephalicus dorsalis and medius.
TS20: thalamus	Present	Regionally restricted	6e	Expressed in ependymal layers of epithalamus and dorsal thalamus and in orthogonal layers of cells immediately above and below the sulcus diencephalicus medius.

Structure	Level	Pattern	Image	Note
TS20: nasal epithelium	Present		7a	Expressed in both the lateral and medial walls.
TS20: olfactory epithelium	Present		7a

Structure	Level	Pattern	Image	Note
TS16: optic stalk	Present		7c , 7c'

Structure	Level	Pattern	Image	Note
TS20: eye anterior segment	Present	Regionally restricted	7d' , 7d	Expressed in the iris, in a peripheral region including ciliary bodies.
TS20: eye	Present	Regionally restricted	7d , 7d'	Expressed in sclera.
TS20: optic nerve	Present	Regionally restricted	7d , 7d'	Expressed in the external ectoderm and the external sheath.

Structure	Level	Pattern	Image	Note
TS25: iris	Present		7e , 7e'

Structure	Level	Pattern	Image	Note
TS16: otocyst	Present		7i

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present		7j
TS20: saccule epithelium	Present		7j

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present		8a/a'
TS20: pharynx epithelium	Present		8a/a'

Structure	Level	Pattern	Image	Note
TS25: oral epithelium	Present		8b/b'
TS25: pharynx epithelium	Present		8b/b'
TS25: thyroglossal duct	Present		8b/b'

Structure	Level	Pattern	Image	Note
TS25: hair follicle	Present	Regionally restricted	8c/c'	Expressed in hair follicles in the jaw.

Structure	Level	Pattern	Image	Note
TS23: brain	Present			Expression remains essentially the same up to E15 embryos and progressively declines later on.