Antibody:	Anti-YAP1 (H00010413-M01)
Detection system:	Secondary antibody coupled to Alexa Fluor 546

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Strong		2A growing oocyte	Strong signal was detected in the cytoplasm with little or no fluorescence detectable in oocyte nuclei.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Strong		2A fully grown oocyte	Strong signal was detected in the cytoplasm with little or no fluorescence detectable in oocyte nuclei.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		2B granulosa-ooctye complex	Signal was detected in the cytoplasm but not in the nucleus.
TS28: ovary follicle cell	Present		2B granulosa-ooctye complex	Signal was detected in the cytoplasm but not in the nucleus.

Structure	Level	Pattern	Image	Note
TS28: cumulus oophorus	Present		2B cumulus-oocyte complex	Signal was detected in the cytoplasm but not in the nucleus.
TS28: oocyte	Present		2B cumulus-oocyte complex	Signal was detected in the cytoplasm but not in the nucleus.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		2D Nf2+/+	Signal was detected in the cytoplasm but remained largely excluded from the nucleus.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		2D Nf2-/-	As in wild type, signal was detected in the cytoplasm but remained largely excluded from the nucleus.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Absent		S1B
TS4: trophectoderm	Present		S1B	Nuclei of trophectodermal cells were positive.