Gene Expression Data
Assay Details
Assay
|
Reference:
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J:214712
Le Bras B, et al., In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014 Jul;219(4):1433-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5828209
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Gene symbol:
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Ank3
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Gene name:
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ankyrin 3, epithelial
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Antibody:
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anti-AnkG
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Detection system:
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Secondary antibody coupled to colloidal gold
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Assay notes:
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Electron microscopy. Fixative was 0.2% glutaraldehyde, 2% paraformaldehyde. Gold particles were increased in size by silver enhancement. Vibratome sections were immunolabeled, post-fixed, and embedded in epon resin for ultrathin sectioning.
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|
Results
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Specimen
4a:
embryonic day 11.5
(more )
Note: (b) is an enlargement of the box in (a).
Specimen
4a:
(close )
Genetic Background:
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CRL:OF1
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Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Resin/plastic
|
Note:
|
(b) is an enlargement of the box in (a).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
4a
,
4b
|
Sub-membraneous localization in axons from the ventral horn.
|
Specimen
4c:
embryonic day 13.5
(more )
Note: (d,e) are enlargements of the boxes in (c,d) respectively.
Specimen
4c:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Resin/plastic
|
Note:
|
(d,e) are enlargements of the boxes in (c,d) respectively.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: ventral grey horn |
Present |
Regionally restricted |
4d
,
4c
,
4e
|
Sub-membraneous localization in axons from the ventral horn.
|
|