Gene Expression Data
Assay Details
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Assay
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Reference:
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J:169049
Varelas X, et al., The Crumbs complex couples cell density sensing to Hippo-dependent control of the TGF-beta-SMAD pathway. Dev Cell. 2010 Dec 14;19(6):831-44
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5828175
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Gene symbol:
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Smad3
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Gene name:
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SMAD family member 3
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Antibody:
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anti-phospho-Smad2/3 (9510)
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Detection system:
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Secondary antibody
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Assay notes:
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This antibody detects endogenous levels of phosporylated Smad2 and Smad3.
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Results
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Specimen
2C:
embryonic day 5.5
(more  )
Note: pre-gastrula stage embryo
Specimen
2C:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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pre-gastrula stage embryo
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: epiblast |
Strong |
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2C p-SMAD2/3 merge
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2C p-SMAD2/3
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Expression is throughout and is prominent in the cytoplasm.
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| TS8: extraembryonic visceral endoderm |
Strong |
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2C p-SMAD2/3 merge
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2C p-SMAD2/3
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Expression is nuclear.
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Analysis Tools
