Probe:	mCad8-PCR
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: brain	Present		3a
TS20: pons	Present		3a
TS20: spinal cord	Present		3a
TS20: thalamus	Present	Regionally restricted	3a	Expressed in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: thymus primordium	Strong		4a

Structure	Level	Pattern	Image	Note
TS22: esophagus	Strong		4b
TS22: thymus primordium	Strong		4b
TS22: trachea	Strong		4b

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	5a	Expressed in the ventral thalamus, in particular regions of the intermediate and mantle layers where early neurons aggregate into brain nuclei and differentiate.
TS20: thalamus mantle layer	Present	Regionally restricted	5a	Expressed in the ventral thalamus.
TS20: thalamus ventricular layer	Present	Regionally restricted	5a	Expressed in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	5e	Expressed in the ventral thalamus, in particular regions of the intermediate and mantle layers where early neurons aggregate into brain nuclei and differentiate.
TS20: thalamus mantle layer	Present	Regionally restricted	5e	Expressed in the ventral thalamus.
TS20: thalamus ventricular layer	Present	Regionally restricted	5e	Expressed in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		5f
TS20: ganglionic eminence	Present	Regionally restricted	5f	Expressed in the ventricular layer ubiquitously.
TS20: telencephalon	Present	Regionally restricted		Expressed in the caudatopallial angle. There is a sharp border of expression between the cells at the caudato-pallial angle and the intermediate zone of the lateral ganglionic eminence and is contiguous with that of early parietal cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		6b
TS20: medial ganglionic eminence	Present	Regionally restricted	6b	Expressed in the ventricular layer ubiquitously. Low expression in the mantle zone of the medial ganglionic eminence.
TS20: telencephalon	Present	Regionally restricted	6b	Expressed in the caudatopallial angle. There is a sharp border of expression between the cells at the caudato-pallial angle and the intermediate zone of the lateral ganglionic eminence and is contiguous with that of early parietal cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		6c
TS20: telencephalon	Present	Regionally restricted	6c	Expressed in the caudatopallial angle. There is a sharp border of expression between the cells at the caudato-pallial angle and the intermediate zone of the lateral ganglionic eminence and is contiguous with that of early parietal cortex.
TS20: medial ganglionic eminence	Present	Regionally restricted	6c	Expressed in the ventricular layer ubiquitously. Low expression in the mantle zone of the medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		7a

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	7b	Expressed in a streak of cells extending from the midventricular zone to the pial surface, where they are especially prominent.
TS20: pons	Present		7b	Moderate to high levels.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	7c	Expressed in a streak of cells extending from the midventricular zone to the pial surface, where they are especially prominent.

Structure	Level	Pattern	Image	Note
TS22: brain nucleus	Present	Regionally restricted	7d	Expressed in the anlage of the facial nucleus and pontine nucleus.

Structure	Level	Pattern	Image	Note
TS22: brain nucleus	Present	Regionally restricted	7e	Expressed in the anlage of the facial nucleus.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	8a	Expressed in the anlage of the pontine nucleus.

Structure	Level	Pattern	Image	Note
TS20: hindbrain mantle layer	Weak	Regionally restricted	8b	Expressed in a thin cord of cells that extends along the longitudinal axis of the hindbrain from the pons to the spinal cord, located in the lateral mantle zone in close proximity to the ventricular layer. Also expressed in another small bilateral cell cord, medially to the previous cord.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Weak	Regionally restricted	8c	Expressed in a thin cord of cells that extends along the longitudinal axis of the hindbrain from the pons to the spinal cord. Located in the lateral mantle zone in close proximity to the ventricular layer.

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS18: embryo	Absent

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted		Expressed in the ventricular and mantle layers of the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted		Expressed in the ventricular and mantle layers of the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: midbrain roof plate	Weak	Regionally restricted		Expressed in the pretectal area.

Structure	Level	Pattern	Image	Note
TS22: medial ganglionic eminence	Weak	Regionally restricted		Low expression in the mantle zone of the medial ganglionic eminence.
TS22: tectum	Weak