Probe:	Hlc cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	1B	Expression is in the splanchnic mesoderm caudal to the level of the anterior intestinal portal and heart with an anterior border at the position of the future midgut/hindgut junction.
TS15: secondary heart field	Absent		1B

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	1C	Expression is in the splanchnic mesoderm caudal to the level of the anterior intestinal portal and heart with an anterior border at the position of the future midgut/hindgut junction.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present	Regionally restricted	1D	Expression is in the splanchnic mesoderm caudal to the level of the anterior intestinal portal and heart with an anterior border at the position of the future midgut/hindgut junction.

Structure	Level	Pattern	Image	Note
TS17: midgut mesenchyme	Present	Regionally restricted	1F	Expression has an anterior boundary of expression within the duodenal region at the level of the liver diverticulum.

Structure	Level	Pattern	Image	Note
TS17: gut mesenchyme	Present		1G
TS17: gut mesentery	Present		1G	Expression is in dorsal mesentery.
TS17: gut endoderm	Absent		1G

Structure	Level	Pattern	Image	Note
TS19: hindgut mesenchyme	Present		1H
TS19: lung	Present		1H
TS19: midgut mesenchyme	Present		1H

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Strong	Regionally restricted	2B	Expression is strong in the mesenchyme surrounding the liver, gall bladder, and intestine. Absent in mesenchyme surrounding the stomach, oesophagus, trachea, and lung bronchi.
TS17: gut mesentery	Strong		2B
TS17: lung mesenchyme	Present		2B
TS17: esophagus	Absent		2B
TS17: stomach	Absent		2B

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Strong	Regionally restricted	2C	Expression is strong in the mesenchyme surrounding the liver, gall bladder, and intestine.
TS17: gut mesentery	Strong		2C
TS17: duodenum	Present	Regionally restricted	2C	The anterior boundary of expression is within the duodenum, just caudal to the stomach constriction.
TS17: foregut-midgut junction mesenchyme	Present		2C
TS17: lung mesenchyme	Present		2C
TS17: midgut mesenchyme	Present		2C
TS17: intraembryonic coelom	Present	Regionally restricted	2C	Expression is in the lining of the coelomic and pericardial cavities.
TS17: pericardial cavity	Present	Regionally restricted	2C	Expression is in the lining of the coelomic and pericardial cavities.
TS17: esophagus	Absent		2C
TS17: stomach	Absent		2C

Structure	Level	Pattern	Image	Note
TS20: liver	Strong		2E
TS20: duodenum	Present	Regionally restricted	2E	The anterior border of expression is within the duodenum.
TS20: gut	Present	Regionally restricted	2E	The anterior border of expression is within the duodenum.
TS20: lung	Weak		2E
TS20: esophagus	Absent		2E
TS20: stomach	Absent		2E

Structure	Level	Pattern	Image	Note
TS20: liver	Strong		2F
TS20: duodenum	Present	Regionally restricted	2F	The anterior border of expression is within the duodenum.
TS20: gut	Present	Regionally restricted	2F	The anterior border of expression is within the duodenum.
TS20: rib pre-cartilage condensation	Present	Regionally restricted	2F	Expression is at the periphery with little or no expression in the chondrogenic condensations.
TS20: lung mesenchyme	Weak		2F
TS20: esophagus	Absent		2F
TS20: pancreas mesenchyme	Absent		2F
TS20: stomach	Absent		2F

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch	Present	Regionally restricted	4B	Expression is in loosely arranged cells.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	4C	Expression is in compact clusters centrally located.
TS17: 2nd branchial arch	Present	Regionally restricted	4C	Expression is in compact clusters centrally located.
TS17: 3rd branchial arch	Present	Regionally restricted	4C	Expression is in compact clusters centrally located.
TS17: 4th branchial arch	Present	Regionally restricted	4C	Expression is in compact clusters centrally located.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	5A	Expression is in dorsal and ventral myogenic masses.

Structure	Level	Pattern	Image	Note
TS19: rib pre-cartilage condensation	Present		6H
TS19: vertebral cartilage condensation	Present	Regionally restricted	6H	Expression is in the neural arches.
TS19: intraembryonic coelom	Present	Regionally restricted	6H	Expression is in the lining of the coelomic and pericardial cavities.
TS19: pericardial cavity	Present	Regionally restricted	6H	Expression is in the lining of the coelomic and pericardial cavities.

Structure	Level	Pattern	Image	Note
TS22: vibrissa dermal component	Present	Regionally restricted	7F	Expression is in a dermal sheath immediately adjacent to the outer root sheath, proximal to the epidermis, and descending to the level of the sebaceous glands.

Structure	Level	Pattern	Image	Note
TS22: vibrissa dermal component	Present	Regionally restricted	7G	Expression is in a dermal sheath immediately adjacent to the outer root sheath, proximal to the epidermis, and descending to the level of the sebaceous glands.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Strong	Regionally restricted		Expreession is caudal to the heart.
TS15: branchial arch	Moderate	Regionally restricted		Expression is in myogenic cells.

Structure	Level	Pattern	Image	Note
TS17: septum transversum	Strong	Regionally restricted		Expression is surrounding the liver diverticulum.
TS17: liver	Strong	Regionally restricted		Expression is in the capsule and endothelial cells.
TS17: gallbladder primordium	Strong	Regionally restricted		Expression is in the mesenchyme.
TS17: duodenum	Strong	Regionally restricted		Expression is in the mesenchyme caudal (midgut) segment.
TS17: hindgut mesenchyme	Strong
TS17: lung mesenchyme	Strong
TS17: midgut mesenchyme	Strong
TS17: branchial arch	Moderate	Regionally restricted		Expression is in myogenic cells.
TS17: intraembryonic coelom	Moderate	Regionally restricted		Expression is in the lining opposite the forelimb bud, adjacent to lung buds and liver.
TS17: sclerotome	Weak	Regionally restricted		Expression is in the latero-caudal region, progenitors of ribs and neural arches.
TS17: brain	Weak	Regionally restricted		Expression is in the meningeal epithelium.
TS17: future spinal cord	Weak	Regionally restricted		Expression is in the meningeal epithelium.

Structure	Level	Pattern	Image	Note
TS19: liver	Strong	Regionally restricted		Expression is in the capsule and endothelial cells.
TS19: gallbladder	Strong	Regionally restricted		Expression is in the mesenchyme.
TS19: duodenum mesenchyme	Strong	Regionally restricted		Expression is in the caudal (midgut) segment.
TS19: hindgut mesenchyme	Strong
TS19: midgut mesenchyme	Strong
TS19: intraembryonic coelom	Strong	Regionally restricted		Expression is in the connective tissue of the diaphragm.
TS19: lung mesenchyme	Moderate
TS19: pericardial cavity	Moderate
TS19: limb	Moderate	Regionally restricted		Expression is in myogenic cells, periosteal regions, and interdigital spaces.
TS19: sclerotome	Moderate	Regionally restricted		Expression is in the latero-caudal region, progenitors of ribs and neural arches.
TS19: branchial arch	Weak	Regionally restricted		Expression is in myogenic cells.
TS19: brain	Weak	Regionally restricted		Expression is in the meningeal epithelium.
TS19: future spinal cord	Weak	Regionally restricted		Expression is in the meningeal epithelium.

Structure	Level	Pattern	Image	Note
TS20: liver	Moderate	Regionally restricted		Expression is in the capsule and endothelial cells.
TS20: gallbladder	Moderate	Regionally restricted		Expression is in the mesenchyme.
TS20: duodenum mesenchyme	Moderate	Regionally restricted		Expression is in the caudal (midgut) segment.
TS20: hindgut mesenchyme	Moderate
TS20: midgut mesenchyme	Moderate
TS20: pericardial cavity	Moderate
TS20: diaphragm	Moderate	Regionally restricted		Expression is in the connective tissue.
TS20: sclerotome	Moderate	Regionally restricted		Expression is in the latero-caudal region, progenitors of ribs and neural arches.
TS20: brain meninges	Weak
TS20: limb interdigital region	Weak
TS20: lung mesenchyme	Weak
TS20: limb	Weak	Regionally restricted		Expression is in myogenic cells and in periosteal regions.
TS20: meninges	Weak	Regionally restricted		Expression is in the meningeal epithelium of the spinal cord.

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Moderate	Regionally restricted		Expression is in the dermal sheath.
TS22: face mesenchyme	Moderate	Regionally restricted		Expression is in the snout mesenchyme.
TS22: liver	Weak	Regionally restricted		Expression is in the capsule and endothelial cells.
TS22: gallbladder	Weak	Regionally restricted		Expression is in the mesenchyme.
TS22: duodenum mesenchyme	Weak	Regionally restricted		Expression is in the caudal (midgut) segment.
TS22: diaphragm	Weak	Regionally restricted		Expression is in the connective tissue.
TS22: hindgut mesenchyme	Weak
TS22: limb interdigital region	Weak
TS22: midgut mesenchyme	Weak
TS22: pericardial cavity	Weak
TS22: limb	Weak	Regionally restricted		Expression is in myogenic cells and in periosteal regions.
TS22: sclerotome	Weak	Regionally restricted		Expression is in the latero-caudal region, progenitors of ribs and neural arches.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS20: diaphragm	Present
TS20: intestine mesenchyme	Present
TS20: hindlimb mesenchyme	Present	Regionally restricted		Expression is in dorsal and ventral myogenic masses.

Structure	Level	Pattern	Image	Note
TS22: diaphragm	Present
TS22: intestine mesenchyme	Present
TS22: rib cartilage condensation	Present	Regionally restricted		Expression is at the periphery with little or no expression in the chondrogenic condensations.
TS22: limb mesenchyme	Weak	Regionally restricted		Expression is in peri-muscular regions.
TS22: cranial muscle	Absent