About   Help   FAQ
Bdnfem1(cre)Zak
Endonuclease-mediated Allele Detail
Summary
Symbol: Bdnfem1(cre)Zak
Name: brain derived neurotrophic factor; endonuclease-mediated mutation 1, Zachary Knight
MGI ID: MGI:5824836
Synonyms: BDNF-2A-Cre
Gene: Bdnf  Location: Chr2:109505045-109557352 bp, + strand  Genetic Position: Chr2, 56.63 cM
Alliance: Bdnfem1(cre)Zak page
Mutation
origin
Strain of Origin:  FVB/NJ
Mutation
description
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
 
Bdnfem1(cre)Zak expression driven by 1 gene
 
Mutation detailsThe bicistronic BDNF-2A-Cre knock-in allele was created using homologous recombination (aided by targeted CRISPR/cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (cre) just upstream of the endogenous STOP codon of the brain derived neurotrophic factor locus (Bdnf). The targeting vector, Cas9 endonuclease and single guide RNA were mixed and injected into the pronuclei of FVB/NJ fertilized zygotes. Embryos were transferred to pseudopregnant females, and correctly targeted pups were bred to C57BL/6J inbred mice for germline transmission. (J:239060)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Bdnf (mouse)
Summary of all recombinase alleles driven by Bdnf.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Bdnf Mutation:  38 strains or lines available
References
Original:  J:239060 Tan CL, et al., Warm-Sensitive Neurons that Control Body Temperature. Cell. 2016 Sep 22;167(1):47-59.e15
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory