Gja4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:62767 Haefliger JA, et al., Connexin37 in normal and pathological development of mouse heart and great arteries. Dev Dyn. 2000 Jun;218(2):331-44
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5824526
Gene symbol:	Gja4
Gene name:	gap junction protein, alpha 4

Antibody:	Cx37 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

4A ©

Specimen 4A: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: foramen ovale of interatrial septum	Present		4A
TS22: right atrium endocardial lining	Present	Regionally restricted	4A	Expression is in some but not all cells.
TS22: right ventricle endocardial lining	Weak		4A

4B ©

Specimen 4B: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: atrioventricular endocardial cushion	Present	Regionally restricted	4B	Expression is in some endocardial cells.

5A ©

Specimen 5A: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: right ventricle cardiac muscle	Present	Regionally restricted	5A	Expression is in the trabecular zone and is weak in the compact zone.

5C ©

Specimen 5C: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: interventricular septum	Absent		5C

7A ©

Specimen 7A: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: outflow tract	Present	Regionally restricted	7A	Expression is strong in endocardial cells and weak in mesenchyme of the conotruncus.

7B ©

Specimen 7B: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: aorta	Present	Regionally restricted	7B	Expression is in the endothelial cells and adventitium and is absent in the media of the ascending aorta.

7C ©

Specimen 7C: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: descending aorta	Present	Regionally restricted	7C	Expression is in the adventitium and is weak in endothelium and media cells.

Specimen Table 1 13 ed.: embryonic day 12.5 (more

)

Structure	Level	Pattern	Note
TS20: heart atrium	Present
TS20: ventricle endocardial lining	Present
TS20: atrioventricular endocardial cushion	Present		Expression is in endocardial cells and is barely detectable in mesenchyme.
TS20: ventricle cardiac muscle	Present		Expression is in the trabecular zone and is barely detectable in the compact zone.
TS20: outflow tract	Present	Regionally restricted	Expression is in the mesenchyme and endocardium of the conotruncal ridges.
TS20: ascending aorta	Present	Regionally restricted	Expression is in the endothelium and is barely detetcable in the media and adventitium.
TS20: artery	Trace	Regionally restricted	Expression is in the endothelium, adventitium and media of the descending aorta.
TS20: interventricular septum	Absent

Specimen Table 1 14 ed.: embryonic day 13.5 (more

)

Structure	Level	Pattern	Note
TS21: heart atrium	Present
TS21: ventricle endocardial lining	Present
TS21: atrioventricular endocardial cushion	Present		Expression is in endocardial cells and is barely detectable in mesenchyme.
TS21: ventricle cardiac muscle	Present		Expression is in the trabecular zone and is barely detectable in the compact zone.
TS21: outflow tract	Present	Regionally restricted	Expression is in the endocardium and is weak in the mesenchyme of the conotruncal ridges.
TS21: ascending aorta	Trace	Regionally restricted	Expression is in the endothelium and adventitium and is absent in the media.
TS21: artery	Trace	Regionally restricted	Expression is in the endothelium, adventitium and media of the descending aorta.
TS21: interventricular septum	Absent

Specimen Table 1 15 ed.: embryonic day 14.5 (more

)

Structure	Level	Pattern	Note
TS22: heart atrium	Present
TS22: ventricle endocardial lining	Present
TS22: atrioventricular endocardial cushion	Present	Regionally restricted	Expression is in endocardial cells and is absent in mesenchyme.
TS22: ventricle cardiac muscle	Present		Expression is in the trabecular zone and is barely detectable in the compact zone.
TS22: heart septum	Present	Regionally restricted	Expression is in the endocardial lining of the interventricular septum, absent everywhere else in the septum.
TS22: outflow tract	Trace	Regionally restricted	Expression is in the mesenchyme and endocardium of the conotruncal ridges.
TS22: ascending aorta	Trace	Regionally restricted	Expression is in the endothelium and adventitium and is absent in the media.
TS22: artery	Trace	Regionally restricted	Expression is in the endothelium, adventitium and media of the descending aorta.
TS22: interventricular septum	Absent

Specimen Table 1 16 ed.: embryonic day 15.5 (more

)

Structure	Level	Pattern	Note
TS23: heart atrium	Present
TS23: ventricle endocardial lining	Present
TS23: atrioventricular endocardial cushion	Present	Regionally restricted	Expression is in endocardial cells and is absent in mesenchyme.
TS23: ventricle cardiac muscle	Trace		Expression is in the trabecular and compact zones.
TS23: outflow tract	Trace	Regionally restricted	Expression is in the mesenchyme and endocardium of the conotruncal ridges.
TS23: ascending aorta	Trace	Regionally restricted	Expression is in the endothelium and adventitium and is absent in the media.
TS23: descending aorta	Trace
TS23: interventricular septum	Absent

Specimen Table 1 17 ed.: embryonic day 16.5 (more

)

Structure	Level	Pattern	Note
TS24: heart atrium	Present
TS24: ventricle endocardial lining	Present
TS24: atrioventricular endocardial cushion	Present	Regionally restricted	Expression is in endocardial cells and is absent in mesenchyme.
TS24: ventricle cardiac muscle	Trace	Regionally restricted	Expression is in the trabecular zone.
TS24: outflow tract	Trace	Regionally restricted	Expression is in the endocardium and is absent in the mesenchyme of the conotruncal ridges.
TS24: ascending aorta	Trace	Regionally restricted	Expression is in the endothelium and adventitium and is absent in the media.
TS24: descending aorta	Trace	Regionally restricted	Expression is in the endothelium and adventitium and is absent in the media.
TS24: interventricular septum	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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