Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33741
Trasler JM, et al., DNA methyltransferase in normal and Dnmtn/Dnmtn mouse embryos. Dev Dyn. 1996 Jul;206(3):239-47
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5824326
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Gene symbol:
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Dnmt1
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Gene name:
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DNA methyltransferase 1
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Antibody:
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anti-DNMT1 (pATH52)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Texas Red
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Results
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Specimen
1B,E,F:
embryonic day 5.5
(more  )
Specimen
1B,E,F:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: embryo |
Present |
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1E
,
1B
,
1F
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Staining was within the nuclei.
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| TS28: decidua |
Present |
Regionally restricted |
1B
,
1E
,
1F
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Expression was not detected in the decidual tissue closest to the embryo. Distal decidual tissue did contain staining.
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Specimen
1D:
embryonic day 5.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: embryo |
Present |
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1D
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Staining was within the nuclei.
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| TS28: decidua |
Present |
Regionally restricted |
1D
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Expression was not detected in the decidual tissue closest to the embryo. Distal decidual tissue did contain staining.
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Specimen
2A,B:
embryonic day 7.5
(more )
Specimen
2A,B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
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2B
,
2A
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Expression was detected in all layers of the embryo. Staining was within the nuclei.
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| TS11: extraembryonic endoderm |
Present |
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2A
,
2B
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| TS28: decidua |
Present |
Regionally restricted |
2B
,
2A
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Expression was detected in the outer decidual cells. The inner decidual zone surrounding the embryo was not stained.
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Specimen
not shown:
embryonic day 7.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Specimen
5A,B:
embryonic day 9.5
(more )
Specimen
5A,B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Widespread |
5A
,
5B
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Expression was abundantly expressed in the nuclei of cells in all tissues of the developing postimplantation embryo.
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| TS15: central nervous system |
Strong |
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5A
,
5B
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High expression levels were noted in the neural tube.
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Dnmt1tm1Jae/Dnmt1tm1Jae
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Ste. Marie's
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Present |
Widespread |
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Analysis Tools