Scube1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226028 Lewandowski JP, et al., Spatiotemporal regulation of GLI target genes in the mammalian limb bud. Dev Biol. 2015 Oct 1;406(1):92-103
Assay type:	RNA in situ
MGI Accession ID:	MGI:5823711
Gene symbol:	Scube1
Gene name:	signal peptide, CUB domain, EGF-like 1

Probe:	Scube1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

S1A Scube1 - E10.5 ©

Specimen S1A - Scube1 - E10.5: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Scube1 - E10.5	Staining is observed in multiple domains.

S1A Scube1 - E11.5 ©

Specimen S1A - Scube1 - E11.5: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Scube1 - E11.5	Staining is observed in multiple domains.

Scube1_a61_E10.5a_RF_JL ©

Specimen Scube1_a61_E10.5a_RF_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Scube1_a61_E10.5a_RF_JL	Staining is observed in multiple domains.

Scube1_a61_E10.5a_RF-ap_JL ©

Specimen Scube1_a61_E10.5a_RF-ap_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Scube1_a61_E10.5a_RF-ap_JL	Staining is observed in multiple domains.

Scube1_a61_E10.5a_RF-dp_JL ©

Specimen Scube1_a61_E10.5a_RF-dp_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Scube1_a61_E10.5a_RF-dp_JL	Staining is observed in multiple domains.

Scube1_a61_E11.5a_RF_JL ©

Specimen Scube1_a61_E11.5a_RF_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Scube1_a61_E11.5a_RF_JL	Staining is observed in multiple domains.

Scube1_a61_E11.5a_RF-ap_JL ©

Specimen Scube1_a61_E11.5a_RF-ap_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Scube1_a61_E11.5a_RF-ap_JL	Staining is observed in multiple domains.

Scube1_a61_E11.5a_RF-dp_JL ©

Specimen Scube1_a61_E11.5a_RF-dp_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Scube1_a61_E11.5a_RF-dp_JL	Staining is observed in multiple domains.

Specimen Scube1_a61_E11.5b_RF_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Scube1_a61_E11.5b_RF_JL	Staining is observed in multiple domains.

Specimen Scube1_a61_E10.5a_JL: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: somite	Strong		Scube1_a61_E10.5a_JL
TS17: branchial arch	Moderate		Scube1_a61_E10.5a_JL

Specimen Scube1_a61_E11.5a_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Scube1_a61_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the somites and branchial arch.

Specimen Scube1_a61_E11.5b_JL: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Scube1_a61_E11.5b_JL	Staining is similar to that observed at E10.5. At that time it was seen in the somites and branchial arch.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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