Probe:	Dlk1 cDNA7
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	2C Dlk1 - E10.5	This gene exhibits category 3 expression: staining is observed in the posterior-proximal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2C Dlk1 - E11.5	This gene exhibits category 3 expression: staining is observed in the posterior-proximal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	S1A Dlk1 - E10.5	This gene exhibits category 3 expression: staining is observed in the posterior-proximal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	S1A Dlk1 - E11.5	This gene exhibits category 3 expression: staining is observed in the posterior-proximal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Dlk1_a14_E10.5a_RF_JL	Staining is observed in the posterior-proximal limb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	Dlk1_a14_E10.5a_RF-pd_JL	Staining is observed in the posterior-proximal limb bud.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Dlk1_a14_E11.5a_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Dlk1_a14_E11.5a_RF-ap_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Dlk1_a14_E11.5a_RF-dp_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Dlk1_a14_E11.5b_RF_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	Dlk1_a14_E11.5b_RF-ap_JL	Domains of staining are similar to those seen at E10.5.

Structure	Level	Pattern	Image	Note
TS17: heart	Strong		Dlk1_a14_E10.5a_JL
TS17: somite	Strong		Dlk1_a14_E10.5a_JL
TS17: branchial arch	Strong		Dlk1_a14_E10.5a_JL
TS17: trunk	Strong		Dlk1_a14_E10.5a_JL	Expression was detected in the presomitic mesoderm and/or trunk region.
TS17: central nervous system	Strong		Dlk1_a14_E10.5a_JL

Structure	Level	Pattern	Image	Note
TS17: heart	Strong		Dlk1_a14_E10.5b_JL
TS17: somite	Strong		Dlk1_a14_E10.5b_JL
TS17: branchial arch	Strong		Dlk1_a14_E10.5b_JL
TS17: trunk	Strong		Dlk1_a14_E10.5b_JL	Expression was detected in the presomitic mesoderm and/or trunk region.
TS17: central nervous system	Strong		Dlk1_a14_E10.5b_JL

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Dlk1_a14_E11.5a_JL	Staining is similar to that observed at E10.5. At that time it was seen in the heart, somites, branchial arch, central nervous system, and presomitic mesoderm and/or trunk.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	Dlk1_a14_E11.5b_JL	Staining is similar to that observed at E10.5. At that time it was seen in the heart, somites, branchial arch, central nervous system, and presomitic mesoderm and/or trunk.