Gene Expression Data
Assay Details
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Assay
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Reference:
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J:226028
Lewandowski JP, et al., Spatiotemporal regulation of GLI target genes in the mammalian limb bud. Dev Biol. 2015 Oct 1;406(1):92-103
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5823571
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Gene symbol:
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Alx4
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Gene name:
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aristaless-like homeobox 4
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Probe:
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Alx4 cDNA12
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
Supplemental Table 1 - Alx4:
embryonic day 10.5
(more  )
Specimen
Supplemental Table 1 - Alx4:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
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Staining is observed in the proximal-anterior limb bud.
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Specimen
Supplemental Data - Alx4:
embryonic day 11.5
(more )
Specimen
Supplemental Data - Alx4:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
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Domains of staining are similar to those seen at E10.5.
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Analysis Tools
