Probe:	IMAGE clone 349651
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Two probes were described MGI:348244 and MGI:221451. Authors indicate that both probes gave identical results, but the probe used in this assay was not specified.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: tail surface ectoderm	Present		4A/B	Signal was detected with a unilateral distribution (corresponding to the right side).
TS17: tail mesenchyme	Present	Regionally restricted	4A/B	Expression was detected in the superficial mesenchyme surrounding the caudal tail region.
TS17: branchial arch ectoderm	Present		4A/B
TS17: branchial arch mesenchyme	Present	Regionally restricted	4A/B	Expression was detected in the superficial mesenchyme in the most posterior branchial arches.

Structure	Level	Pattern	Image	Note
TS17: tail surface ectoderm	Present		4C/D	Signal was detected with a unilateral distribution (corresponding to the right side).
TS17: tail mesenchyme	Present	Regionally restricted	4C/D	Expression was detected in the superficial mesenchyme surrounding the caudal tail region.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		4G/H , 4E/F	Expression was in dorsal root ganglia of the spinal and cranial regions.
TS20: liver	Strong	Regionally restricted	4G/H , 4E/F	Expression was strongest within the lateral walls in the caudal portions of the liver. Strong expression was detected in liver cells surrounding the hepatic venous plexus.

Structure	Level	Pattern	Image	Note
TS20: liver	Strong	Regionally restricted	4I/J	Expression was strongest within the lateral walls. Strong expression was detected in liver cells surrounding the hepatic venous plexus.

Structure	Level	Pattern	Image	Note
TS20: remnant of Rathke's pouch	Strong	Regionally restricted	4K/L	Expression was strong in the anterior wall of Rathke's pouch.

Structure	Level	Pattern	Image	Note
TS20: tail surface ectoderm	Present	Regionally restricted	4M/N , 4O/P	Expression was detected in the surface ectoderm covering the caudal tail region.
TS20: genital tubercle	Present	Regionally restricted	4O/P , 4M/N	Expression was detected in the inferior wall of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS20: cranium	Strong	Regionally restricted	5A/B	Strong expression was detected in the condensing mesenchyme forming the roofing bones of the skull (parietal bone).

Structure	Level	Pattern	Image	Note
TS22: perichondrium	Weak	Regionally restricted	5C/D	Expression was detected in the perichondrium of the vertebral bodies and associated neural arches.
TS22: Meckel's cartilage	Present	Regionally restricted	5E/F , 5C/D	Expression was detected in the perichondrium of the proximal and distal extremities of Meckel's cartilage.

Structure	Level	Pattern	Image	Note
TS22: cranium	Present	Regionally restricted	5G/H , 5I/J	Expression was detected in the basicranium.
TS22: trigeminal ganglion	Present		5G/H , 5I/J

Structure	Level	Pattern	Image	Note
TS22: perichondrium	Present	Regionally restricted	5K/L	Exression was detected in the perichondrium of the developing rib shafts.

Structure	Level	Pattern	Image	Note
TS22: perichondrium	Present	Regionally restricted	5M/N	Exression was detected in the perichondrium of the appendicular skeleton; it was pronounced in the long bones of the fore- and hindlimbs.

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Present	Regionally restricted	5O/P	Expression was detected in the periotic mesenchyme surrounding the developing inner ear.

Structure	Level	Pattern	Image	Note
TS22: remnant of Rathke's pouch	Absent

Structure	Level	Pattern	Image	Note
TS24: embryo	Weak	Regionally restricted		Expression was largely downregulated throughout the embryo, although it was still detectable in many of the tissues where it was earlier expressed.

Structure	Level	Pattern	Image	Note
TS26: embryo	Absent