Probe:	Nkx3.1 probe E
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Similar results were obtained for each of the three probes (D, E, and F).

Structure	Level	Pattern	Image	Note
TS22: urogenital sinus	Present	Regionally restricted	6A	Expressed in an outbudding that will give rise to the ventral prostate.
TS22: urethra	Present		6A	Expressed at lower levels.

Structure	Level	Pattern	Image	Note
TS22: urogenital sinus	Present	Regionally restricted	6B , 6C	Expressed in an outbudding.
TS22: epithelium of urogenital sinus	Present	Regionally restricted	6C	Highest expression in the cells at the leading edge of the outbudding epithelium.

Structure	Level	Pattern	Image	Note
TS25: ventral prostate bud	Present	Regionally restricted	6D	Expressed bilaterally.

Structure	Level	Pattern	Image	Note
TS22: seminiferous cord	Present		6E	Expressed in the medullary cords, which will later form the seminiferous tubules.

Structure	Level	Pattern	Image	Note
TS25: seminiferous cord	Present		6F
TS25: interstitium of the testis	Absent		6F
TS25: tunica albuginea of testis	Absent		6F

Structure	Level	Pattern	Image	Note
TS25: anterior prostate bud	Present		6G
TS25: dorsal prostate bud	Present		6G
TS25: lateral prostate bud	Present		6G
TS25: ductus deferens	Absent		6G
TS25: urethra epithelium	Absent		6G

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Present	Regionally restricted	6H	Expressed in the endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: renal artery	Present		6I	Expressed in the renal arteries that arise from the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present

Structure	Level	Pattern	Image	Note
TS25: epididymis	Absent
TS25: urothelium of bladder	Absent
TS25: descending aorta	Present