Probe:	Meis2b cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	4A	Authors describe restricted expression in middle region of embryo, up to an ill-defined boundary at base of headfold.

Structure	Level	Pattern	Image	Note
TS12: trunk	Present		4B	Expression restricted to middle (presumptive cervical and trunk) regions.
TS12: neural tube	Present		4B
TS12: future hindbrain	Present	Regionally restricted	4B	Expression extending to anterior rhombencephalon.
TS12: rhombomere	Present	Regionally restricted	4B	Stripe of increased expression corresponding to presumptive rhombomere 3.
TS12: somite	Present	Regionally restricted	4B	Expression in somite mesoderm.

Structure	Level	Pattern	Image	Note
TS13: somite	Strong	Regionally restricted	4C	Increased expression in lateral region of somites.
TS13: rhombomere 3	Strong		4C	Increased expression in developing rhombomere 3.
TS13: 1st branchial arch	Strong		4C	Increased expression in first branchial arch.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord	Present	Regionally restricted	4D	Expression extends posteriorly along the spinal cord.
TS14: future hindbrain	Present	Regionally restricted	4D	Expression in in hindbrain extends up to level of rhombomere 2.
TS14: rhombomere 2	Present		4D	Expression in in hindbrain extends up to level of rhombomere 2.
TS14: future midbrain	Present		4D	Sharply defined expression domain in midbrain.
TS14: future telencephalon	Weak	Regionally restricted	4D	Weak expression in telencephalic neurectoderm.
TS14: 1st branchial arch maxillary component	Strong		4D	Pronounced expression in maxillary region derived from first branchial arch.
TS14: 1st branchial arch maxillary-mandibular groove	Present	Regionally restricted	4D	Expression along each side of maxillo-mandibular cleft.
TS14: branchial groove	Present		4D	Authors report expression along sides of more posterior branchial clefts.
TS14: heart	Strong	Regionally restricted	4D	Strong expression in dorsal regions of the heart.
TS14: trunk mesenchyme	Strong	Regionally restricted	4D	Strong expression at level of forelimb buds. Specific expression in region where hindlimb buds start to appear.
TS14: forelimb bud mesenchyme	Present	Patchy	4D	Expression in trunk mesenchyme extending into forelimb buds.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord	Present	Regionally restricted	4E	Expression extends posteriorly along the spinal cord.
TS14: future hindbrain	Present	Regionally restricted	4E	Expression in in hindbrain extends up to level of rhombomere 2.
TS14: rhombomere 2	Strong		4E	More intense expression in rhombomere 3.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted	4F	Expression in in hindbrain extends up to level of rhombomere 2.
TS14: future midbrain	Present	Regionally restricted	4F	Sharply defined expression domain in midbrain.
TS14: 1st branchial arch maxillary component	Strong		4F	Pronounced expression in maxillary region derived from first branchial arch.
TS14: trunk mesenchyme	Present	Regionally restricted	4F	Specific expression in trunk mesenchyme in region where hindlimb buds start to appear.

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	4G	Increased expression in lateral region of somites.