Probe:	probe 1
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Hybridizations shown in this study were performed with two different probes, probe 1 (MGI:5823007) and probe 2 (MGI:5823008). Authors report that both probes gave identical results. Only those obtained with probe 1 are shown.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Moderate	Regionally restricted	3A	Intermediate levels of expression in isocortex ventricular layer.
TS22: telencephalon septum	Strong	Regionally restricted	3A	Strong expression in incipient septal mantle layer.
TS22: tectum	Present		3A
TS22: midbrain tegmentum	Present	Regionally restricted	3A	Expression in rostral midbrain tegmentum.
TS22: brainstem	Strong	Regionally restricted	3A	Wide areas of intense expression in pontine tegmentum that could be assigned to the differentiating reticular formation.
TS22: cerebellum	Present	Uniform	3A	Homogeneous expression in cerebellar primordium.
TS22: medulla oblongata	Present	Widespread	3A	Widespread areas of expression in medulla tentatively identified as cochlear nuclei, superior olivary complex, principal sensory nucleus of trigeminal, interior olive, and nuclei of reticular formation.

Structure	Level	Pattern	Image	Note
TS22: amygdala	Strong	Regionally restricted	3B	Strong expression in region of differentiating amygdalar mantle layer, with a complicated heterogeneous pattern.
TS22: dorsal thalamus	Absent		3B
TS22: zona limitans intrathalamica	Present		3B	Expression in cells appearing to cross the border between dorsal and ventral thalamus (zona limitans intrathalamica).
TS22: prethalamus	Strong	Regionally restricted	3B	Strong expression in most of ventral thalamus, with exception of a small caudal region.
TS22: hypothalamus	Strong	Regionally restricted	3B	Intense levels of heterogeneous expression in lateral part of hypothalamic mantle. Very strong expression in anterior hypothalamic area, continuous region with ventral thalamus.
TS22: pretectum	Absent		3B

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present		3C	Authors report expression in most of the tectum.
TS22: superior colliculus	Present		3C	Authors report expression in most of the tectum.
TS22: cerebellum	Present	Uniform	3C	Homogeneous expression in cerebellar primordium.
TS22: medulla oblongata	Present	Widespread	3C	Widespread areas of expression in medulla tentatively identified as cochlear nuclei, superior olivary complex, and principal sensory nucleus of trigeminal.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Strong	Regionally restricted		High levels of expression in prospective bed nucleus of stria terminalis.
TS22: cornea epithelium	Moderate			Intermediate levels of expression in ectoderm covering the eye (future eyelids and cornea).
TS22: eyelid epithelium	Moderate			Intermediate levels of expression in ectoderm covering the eye (future eyelids and cornea).
TS22: optic chiasma	Absent
TS22: optic nerve	Absent
TS22: corpus striatum	Absent			No expression in striatal or pallidal primordia.
TS22: pallidum	Absent			No expression in striatal or pallidal primordia.