Gene Expression Data
Assay Details
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Assay
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Reference:
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J:59629
Lehtonen S, et al., Syntaxin 3 and Munc-18-2 in epithelial cells during kidney development. Kidney Int. 1999 Sep;56(3):815-26
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5822841
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Gene symbol:
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Stxbp2
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Gene name:
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syntaxin binding protein 2
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Antibody:
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Anti-Munc-18-2
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Fixative: 3.5% paraformaldehyde.
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Results
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Specimen
6A:
embryonic day 11.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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(CBA x NMRI)F1
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: ureteric bud |
Present |
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6A
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Staining was restricted to the apical plasma membrane domain of the cells. The undifferentiated mesenchymal cells showed no staining.
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Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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(CBA x NMRI)F1
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B:
embryonic day 15.5
(more )
Specimen
6B:
(close )
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Genetic Background:
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(CBA x NMRI)F1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6D:
embryonic day 17.5
(more )
Specimen
6D:
(close )
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Genetic Background:
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(CBA x NMRI)F1
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: collecting duct |
Present |
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6D
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Signal was concentrated apically with a weak cytoplasmic signal, especially in the collecting duct cells.
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| TS25: renal proximal tubule |
Present |
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6D
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Signal was concentrated apically with a weak cytoplasmic signal, especially in the collecting duct cells.
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| TS25: ureteric tip |
Weak |
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6D
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| TS25: pelvic urothelial lining |
Ambiguous |
Regionally restricted |
6D
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Weak or no signal was seen in the epithelial cells of the renal pelvis.
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| TS25: renal cortex |
Ambiguous |
Regionally restricted |
6D
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Weak or no signal was seen in the early differentiating epithelial structures in the renal cortex.
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Specimen
6E:
postnatal adult
(more )
Specimen
6E:
(close )
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Genetic Background:
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NMRI
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal proximal tubule |
Present |
Regionally restricted |
6E
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In the cortex of the adult kidney, expression was localized apically in the proximal tubules.
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Specimen
6G:
postnatal adult
(more )
Specimen
6G:
(close )
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Genetic Background:
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NMRI
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: proximal straight tubule, outer medullary portion |
Present |
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6G
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Expression was prominent in the S3 segment of the proximal tubule. The localization of expression appeared as intracellular structures mainly close to the basolateral pole of the cells.
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