Antibody:	Anti-Syntaxin 3
Detection system:	Secondary antibody coupled to rhodamine
Assay notes:	Fixative: 3.5% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Present		5A	Staining was restricted to the apical plasma membrane domain of the cells. The undifferentiated mesenchymal cells showed no staining.

Structure	Level	Pattern	Image	Note
TS21: collecting duct	Present

Structure	Level	Pattern	Image	Note
TS23: s-shaped body	Weak		5B	Expression localized apically in the developing epithelial cells.
TS23: cortical early proximal tubule	Present		5B
TS23: comma-shaped body	Absent		5B

Structure	Level	Pattern	Image	Note
TS25: collecting duct	Present		5D	Signal was concentrated apically.
TS25: renal proximal tubule	Present		5D	Signal was concentrated apically.
TS25: ureteric tip	Weak		5D
TS25: pelvic urothelial lining	Ambiguous	Regionally restricted	5D	Weak or no signal was seen in the epithelial cells of the renal pelvis.
TS25: renal cortex	Ambiguous	Regionally restricted	5D	Weak or no signal was seen in the early differentiating epithelial structures in the renal cortex.

Structure	Level	Pattern	Image	Note
TS28: renal proximal tubule	Present	Regionally restricted	5E	In the cortex of the adult kidney, expression was localized apically in the convoluted portion of the proximal tubules.

Structure	Level	Pattern	Image	Note
TS28: proximal straight tubule, outer medullary portion	Absent		5G	In the outer medulla, there was a zone in which the expression disappeared. The region corresponds to the S3 segment of the proximal tubule.