Nanog Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:95814 Hatano SY, et al., Pluripotential competence of cells associated with Nanog activity. Mech Dev. 2005 Jan;122(1):67-79
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5819128
Gene symbol:	Nanog
Gene name:	Nanog homeobox

Antibody:	anti-Nanog
Detection system:	Secondary antibody coupled to Alexa Fluor 546
Assay notes:	Specimens were fixed in either 3.7% formaldehyde or 4% paraformaldehyde.

Results

3_MII Oocyte ©

Specimen MII Oocyte: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: oocyte	Absent		3_MII Oocyte

Specimen 2-cell not shown: embryonic day 1.0 (more

)

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Absent

3_8-cell ©

Specimen 8-cell: embryonic day 2.5 (more

)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Absent		3_8-cell

3_Morula ©

Specimen Morula: embryonic day 3.0 (more

)

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		3_Morula

3_Blastocyst ©

Specimen Blastocyst: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		3_Blastocyst
TS4: trophectoderm	Absent		3_Blastocyst

3_E6.5 ©

Specimen E6.5: embryonic day 6.5 (more

)

Structure	Level	Image
TS9: embryo ectoderm	Present	3_E6.5
TS9: embryo mesoderm	Absent	3_E6.5
TS9: extraembryonic endoderm	Absent	3_E6.5

3_E7.0 ©

Specimen E7.0: embryonic day 7.0 (more

)

Structure	Level	Image
TS10: embryo ectoderm	Present	3_E7.0
TS10: embryo mesoderm	Absent	3_E7.0
TS10: extraembryonic endoderm	Absent	3_E7.0

3_E7.5 ©

Specimen E7.5: embryonic day 7.5 (more

)

Structure	Level	Image
TS11: embryo ectoderm	Present	3_E7.5
TS11: embryo mesoderm	Absent	3_E7.5
TS11: extraembryonic endoderm	Absent	3_E7.5

3_E8.0 ©

Specimen E8.0: embryonic day 8.0-8.5 (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent		3_E8.0

3_NANOG ©

3_NANOG-PGC7 ©

Specimen E7.5 NANOG: embryonic day 7.5 (more

)
Note: Double labeled: red - Nanog; green - PGC7/Stella (Dppa3 assay MGI:5819129).

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3_NANOG-PGC7 , 3_NANOG	Expression in caudal region (primitive streak region) of epiblast.
TS11: embryo ectoderm	Present	Graded	3_NANOG , 3_NANOG-PGC7	Intensity of expression gradually decreased from proximal to distal region of epiblast.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24