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Gene Expression Data
Assay Details
Assay
Reference: J:95814 Hatano SY, et al., Pluripotential competence of cells associated with Nanog activity. Mech Dev. 2005 Jan;122(1):67-79
Assay type: RT-PCR
MGI Accession ID: MGI:5819120
Gene symbol: Nanog
Gene name: Nanog homeobox
Probe: Nanog-pS, Nanog-pT
Visualized with: Ethidium bromide
Results
Image: 1F ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) 516 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
M Mol. Wt. Marker Lane
ES - Cultured cells: no data stored
ES + Cultured cells: no data stored
E7.5 - E7.5 TS11: embryo Absent Present Not Specified; poly-A+ RNA Not Specified Not Specified
E7.5 + E7.5 TS11: embryo Present Absent Not Specified; poly-A+ RNA Not Specified Not Specified Amplification products were digested with NiaIII to distinguish Nanog mRNA from that of a pseudogene, Nanog-ps1.
E12.5 GR - E12.5 TS20: urogenital ridge Absent Present Not Specified; poly-A+ RNA Not Specified Not Specified
E12.5 GR + E12.5 TS20: urogenital ridge Present Absent Not Specified; poly-A+ RNA Not Specified Not Specified Amplification products were digested with NiaIII to distinguish Nanog mRNA from that of a pseudogene, Nanog-ps1.
blastocyst - E3.5 (b) TS4: blastocyst Absent Present Not Specified; poly-A+ RNA Not Specified Not Specified
blastocyst + E3.5 (b) TS4: blastocyst Present Absent Not Specified; poly-A+ RNA Not Specified Not Specified Amplification products were digested with NiaIII to distinguish Nanog mRNA from that of a pseudogene, Nanog-ps1.
Notes:
(a) Represents 3 fragments of 161, 173 and 182 bp.
(b) Age assigned by curator based on morphological criteria supplied by authors.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory