Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:231073
Oishi K, et al., Mutually repressive interaction between Brn1/2 and Rorb contributes to the establishment of neocortical layer 2/3 and layer 4. Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3371-6
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5818696
|
Gene symbol:
|
Rorb
|
Gene name:
|
RAR-related orphan receptor beta
|
Antibody:
|
Anti-Rorb
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
Assay notes:
|
Two Rorb antibodies were described MGI:5818683 and MGI:5818684; either could have been used in this assay.
|
|
Results
|
Specimen
1B:
embryonic day 16.5
(more )
Note: Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cortical plate |
Present |
Regionally restricted |
1B Rorb
,
1B Rorb Cux1
|
Expression was mainly restricted to the lower cortical plate; there was no overlap with Cux1 expression at E16.5.
|
Specimen
1D:
postnatal day 1.5
(more )
Note: Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
|
Specimen
S1B:
embryonic day 18.5
(more )
Note: Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
Specimen
S1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cortical plate |
Present |
Regionally restricted |
S1B Rorb
,
S1B Rorb Cux1
|
Expression was mainly restricted to the lower cortical plate; there was little overlap with Cux1 expression at E18.5.
|
Specimen
S1D:
postnatal day 3.5
(more )
Note: Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
Specimen
S1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 3.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Rorb; magenta - Cux1 (assay MGI:5818698).
|
|