Gene Expression Data
Assay Details
Assay
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Reference:
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J:18225
Gaunt SJ, et al., Forward spreading in the establishment of a vertebrate Hox expression boundary: the expression domain separates into anterior and posterior zones, and the spread occurs across implanted glass barriers. Dev Dyn. 1994 Mar;199(3):229-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5818136
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Gene symbol:
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Hoxa3
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Gene name:
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homeobox A3
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Probe:
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Hoxa3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
6E:
embryonic day 8.75
(more )
Note: 15-17 somites.
Specimen
6E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.75
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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15-17 somites.
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Structure |
Level |
Pattern |
Image |
Note |
TS13: primitive streak |
Present |
Regionally restricted |
6E
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The posterior zone of expression was centered on the primitive streak.
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TS13: neural ectoderm |
Present |
Regionally restricted |
6E
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There were posterior zone and anterior zone expression domains, with an intermediate zone that was marked by much weaker, or even absent staining.
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TS13: somite |
Present |
Regionally restricted |
6E
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There were posterior zone and anterior zone expression domains, with an intermediate zone that was marked by much weaker, or even absent staining.
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TS13: rhombomere 5 |
Strong |
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6E
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The anterior boundary of expression in neurectoderm was clearly marked by strong label in rhombomere 5.
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