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Gene Expression Data
Assay Details
Assay
Reference: J:199559 Manning DK, et al., Loss of the ciliary kinase Nek8 causes left-right asymmetry defects. J Am Soc Nephrol. 2013 Jan;24(1):100-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5818024
Gene symbol: Nek8
Gene name: NIMA (never in mitosis gene a)-related expressed kinase 8
Antibody: anti-Nek8
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3I: embryonic day 8.0 (more )
Note: Double labeled: red - Nek8; green - acetylated alpha-tubulin.
Structure Level Pattern Image Note
TS12: node Present Regionally restricted 3I Expressed in all node monocilia at base of cilia.
Specimen 3J: embryonic day 8.0; Nek8tm1Bei/Nek8tm1Bei (more )
Note: Double labeled: red - Nek8; green - acetylated alpha-tubulin.
Structure Level Pattern Image Note
TS12: node Absent 3J Not detected in nodal cilia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory