Gene Expression Data
Assay Details
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Assay
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Reference:
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J:199559
Manning DK, et al., Loss of the ciliary kinase Nek8 causes left-right asymmetry defects. J Am Soc Nephrol. 2013 Jan;24(1):100-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5817985
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Gene symbol:
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Pitx2
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Gene name:
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paired-like homeodomain transcription factor 2
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Probe:
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Pitx2c probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3E:
embryonic day 8.0-8.5
(more  )
Note: 6 somites
Specimen
3E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
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Expressed in the right lateral plate mesoderm in one embryo.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCr
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Mutant Allele(s):
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Nek8tm1Bei/Nek8tm1Bei
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Age:
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embryonic day 8.0-8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
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Expressed in the left lateral plate mesoderm in one embryo.
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Analysis Tools
