Gabpb1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:96483 O'Leary DA, et al., Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1. Gene. 2005 Jan 3;344:79-92
Assay type:	RT-PCR
MGI Accession ID:	MGI:5817583
Gene symbol:	Gabpb1
Gene name:	GA repeat binding protein, beta 1

Probe:	Beta1f, B1-2r
Assay notes:	The RT-PCR products were blotted and hybridized with an internal oligonucleotide probe MGI:5817576.

Results

Image: 6B bottom

	Sample Information		Bands					Other Sample Information
Lane	Age	Structure	928 bp (a)	842 bp (b)	817 bp (c)	734 bp (d)	621 bp (e)	Amount	Genetic Background	Mutant Allele(s)	Sex
Stomach +	P adult	TS28: stomach	Present	Ambiguous	Ambiguous	Ambiguous	Present	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Stomach -	Control
Large Int. +	P adult	TS28: large intestine	Present	Ambiguous	Ambiguous	Ambiguous	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Large Int. -	Control
Small Int. +	P adult	TS28: small intestine	Present	Ambiguous	Ambiguous	Ambiguous	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Small Int. -	Control
Fat +	P adult	TS28: adipose tissue	Present	Ambiguous	Ambiguous	Ambiguous	Present	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Fat -	Control
Sk. mus +	P adult	TS28: skeletal muscle	Strong	Ambiguous	Ambiguous	Ambiguous	Present	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Sk. mus -	Control
Tongue +	P adult	TS28: tongue	Strong	Ambiguous	Ambiguous	Ambiguous	Present	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Tongue -	Control
Eyes +	P adult	TS28: eye	Present	Ambiguous	Ambiguous	Ambiguous	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Not Specified
Eyes -	Control
Testis +	P adult	TS28: testis	Strong	Ambiguous	Ambiguous	Ambiguous	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Male
Testis -	Control
Epidid. +	P adult	TS28: epididymis	Present	Ambiguous	Ambiguous	Ambiguous	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Male
Epidid. -	Control
Ovary/Uterus +	P adult	TS28: ovary TS28: uterus	Present	Ambiguous	Ambiguous	Ambiguous	Absent	Not Specified; poly-A+ RNA	(C57BL/6 x CBA)F1		Female
Ovary/Uterus -	Control

Notes:
(a) Full length transcript. Gabp-beta1-2 isoform.
(b) Splice form - exon 6-8.
(c) Splice form - exon 4-6.
(d) Splice form - exon 3-5.
(e) Splice form - exon 3-6.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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